N-Methylcytisine Ameliorates Dextran-Sulfate-Sodium-Induced Colitis in Mice by Inhibiting the Inflammatory Response

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Yan-Fang JiaoJian-Qiang Yu

Abstract

This study aimed to investigate the anti-inflammatory effects of N-methylcytisine (NMC) in a dextran sulfate sodium (DSS)-induced colitis model and explore its possible mechanisms. Experimental colitis was induced by administering the mice with 5% DSS for 7 days. Different doses of NMC (1, 4 and 16 mg/kg) and 5-aminosalicylic acid (100 mg/kg) were given orally once every day for 7 days. The protective effect of NMC was evaluated using the disease activity index, colon length and results of histopathological examination. The possible mechanisms of NMC were explored by evaluating the expression levels of tumour necrosis factor-α, interleukin-1β and interleukin-6 (IL-6) using ELISA and analysing the protein expression levels of nuclear factor (NF)-κB p65, p-NF-κB p65, p-IκB, IκB, IκB kinase (IKK) and p-IKK using western blots. Results demonstrated that the oral administration of NMC attenuated the DSS-induced clinical symptoms and pathological damage. In addition, NMC treatment significantly reduced myeloperoxidase activity and level of pro-inflammatory cytokines. Further studies revealed that NMC blocked the activation of NF-κB by inhibiting IκB and IKK phosphorylation. These findings suggested that NMC exerts anti-inflammatory e...Continue Reading

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Citations

Jan 24, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hui ShiXiao-Dong Peng
Aug 7, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zhe WangMin Chang
Feb 22, 2021·Journal of Ethnopharmacology·Guo-Hui LiHong-Xiang Lou
Aug 3, 2021·Frontiers in Pharmacology·Linshan DuanGuoyan Liu
Apr 14, 2021·Canadian Journal of Physiology and Pharmacology·Adeola Temitope SalamiSamuel Babafemi Olaleye

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Methods Mentioned

BETA
ELISA
light microscopy
Assay
protein assay
electrophoresis

Software Mentioned

Quantity One

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