PMID: 9174124May 1, 1997Paper

N-myc over-expression downregulates alpha3beta1 integrin expression in human Saos-2 osteosarcoma cells

Clinical & Experimental Metastasis
R Judware, L A Culp

Abstract

Alterations in adhesion to the extracellular matrix mediated by integrin receptors are commonly observed in a wide variety of transformed/tumor classes. Reductions in the expression of several integrin subunits have been documented in human neuroblastoma cell lines that over-express the neuroblastoma-associated oncogene N-myc. Neuroblastoma cells transfected with a cDNA encoding N-myc on a high-expression plasmid exhibit greatly reduced levels of alpha2, alpha3 and beta1 integrin subunits with concomitant rounding of cells on substrata. In the current studies, we examined whether integrin downregulation by N-myc is cell-type specific by transfecting a human N-myc cDNA into Saos-2 human osteosarcoma cells and evaluating integrin expression. Several N-myc-expressing cell lines were isolated which exhibit reduced levels of beta1 integrin subunit protein and significant alteration in cell morphology - these cell lines resemble N-myc-over-expressing neuroblastoma cells. In addition to reduced beta1 subunit levels, the osteosarcoma-derived N-myc transfectants exhibit little or no alpha3beta1 integrin complexes, either intracellular or at the cell surface. Finally, reduced amounts of alpha3 integrin subunit in these cell lines occur a...Continue Reading

Citations

Apr 14, 2019·Proteomes·Stephanie N Hurwitz, David G Meckes
Jan 1, 2005·The Journal of Membrane Biology·Tsutomu Tsuji
May 30, 1998·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·L A CulpR Lechner
Sep 23, 2006·Molecular Therapy : the Journal of the American Society of Gene Therapy·Naseruddin HötiRonald Rodriguez
Mar 27, 1999·Biochemical and Biophysical Research Communications·E Y ShinS Y Kim
Sep 9, 2000·Current Opinion in Cell Biology·J A Kreidberg

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