N-myristoylation and S-acylation are common modifications of Ca2+ -regulated Arabidopsis kinases and are required for activation of the SLAC1 anion channel

The New Phytologist
Shunya SaitoNobuyuki Uozumi

Abstract

N-myristoylation and S-acylation promote protein membrane association, allowing regulation of membrane proteins. However, how widespread this targeting mechanism is in plant signaling processes remains unknown. Through bioinformatics analyses, we determined that among plant protein kinase families, the occurrence of motifs indicative for dual lipidation by N-myristoylation and S-acylation is restricted to only five kinase families, including the Ca2+ -regulated CDPK-SnRK and CBL protein families. We demonstrated N-myristoylation of CDPK-SnRKs and CBLs by incorporation of radiolabeled myristic acid. We focused on CPK6 and CBL5 as model cases and examined the impact of dual lipidation on their function by fluorescence microscopy, electrophysiology and functional complementation of Arabidopsis mutants. We found that both lipid modifications were required for proper targeting of CBL5 and CPK6 to the plasma membrane. Moreover, we identified CBL5-CIPK11 complexes as phosphorylating and activating the guard cell anion channel SLAC1. SLAC1 activation by CPK6 or CBL5-CIPK11 was strictly dependent on dual lipid modification, and loss of CPK6 lipid modification prevented functional complementation of cpk3 cpk6 guard cell mutant phenotypes...Continue Reading

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Citations

Sep 22, 2018·Journal of Experimental Botany·Mukesh Kumar MeenaDebasis Chattopadhyay
Aug 2, 2019·The New Phytologist·Tiffany Yip Delormel, Marie Boudsocq
Nov 7, 2019·Plant Biotechnology Journal·Xiaomin ZhangFeng Qin
Dec 1, 2018·IUBMB Life·Fatemeh MohammadzadehMasoud Darabi
Mar 3, 2020·Frontiers in Plant Science·Shunya Saito, Nobuyuki Uozumi
Apr 5, 2020·Science China. Life Sciences·Zhizhong GongJian-Kang Zhu
Aug 14, 2020·International Journal of Molecular Sciences·Xiao MaZhen-Hui Gong
Jan 6, 2021·Journal of Integrative Plant Biology·Xuexue ChenZhizhong Gong
Apr 3, 2021·Plant Physiology and Biochemistry : PPB·Marcelo Daniel SciorraRita María Ulloa
Jan 12, 2021·Annual Review of Plant Biology·Omar Pantoja
Jul 4, 2021·The Plant Journal : for Cell and Molecular Biology·Saori OgasawaraToshihiko Hayakawa

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