N-protein mobilisation associated with the leaf senescence process in oilseed rape is concomitant with the disappearance of trypsin inhibitor activity

Functional Plant Biology : FPB
Philippe EtienneJean-Christophe Avice

Abstract

Brassica napus L. (oilseed rape) is an important crop plant characterised by low nitrogen (N) use efficiency. This is mainly due to a weak N recycling from leaves that is related to incomplete protein degradation. Assuming that protease inhibitors are involved throughout protein mobilisation, the goal of this study was to determine their role in the control of N mobilisation associated with leaf senescence. Results showed that a 19-kDa polypeptide exhibiting trypsin inhibitor (TI) activity presented an increased gradient from the older to the younger leaves. According to the SAG12/Cab gene expression profile, which is an indicator of leaf senescence, mature leaves of nitrate-deprived plants presented an earlier initiation of senescence and a decrease in protein concentration when compared with nitrate-replete plants. This coincided with disappearance of both TI activity and a reduction in the transcript level of the BnD22 gene (encoding a protein sharing homology with Künitz protease inhibitor). In young leaves of N-deprived plants, initiation of senescence was delayed; soluble protein concentration was maintained while both TI activity and BnD22 transcripts were high. This indicates that in oilseed rape growing under nitrate d...Continue Reading

References

Feb 1, 1996·Plant Molecular Biology·C HanfreyV Buchanan-Wollaston
Mar 1, 1997·Plant Molecular Biology·V Buchanan-Wollaston, C Ainsworth
Feb 24, 2001·Plant Molecular Biology·E P BeersC Zhao
Jul 28, 2001·Physiologia Plantarum·María G. GuevaraClaudia R. Oliva
Mar 26, 2002·Journal of Experimental Botany·Stefan Hörtensteiner, Urs Feller
Dec 24, 2002·Current Opinion in Plant Biology·Satoko Yoshida
Feb 25, 2003·Journal of Experimental Botany·Simon A CoupeJocelyn R Eason
Mar 30, 2004·Plant Physiology·Christiane ReinbotheSteffen Reinbothe
Sep 29, 2005·Journal of Experimental Botany·Nathalie NicotDanièle Evers
Dec 16, 2006·The Journal of Biological Chemistry·Daisuke HorigomeAkira Uchida
Dec 21, 2006·Annual Review of Plant Biology·Pyung Ok LimHong Gil Nam

❮ Previous
Next ❯

Citations

Jul 6, 2019·Frontiers in Plant Science·Agustina BuetJuan J Guiamet
May 3, 2014·Journal of Experimental Botany·Jean-Christophe Avice, Philippe Etienne
Mar 3, 2017·Biochemistry·Daniel M PalmHarald Paulsen
Jun 19, 2010·Biochimie·Meriem BenchabaneDominique Michaud

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.