PMID: 9180144May 29, 1997Paper

N-ras protein: frequent quantitative and qualitative changes occur in human colorectal carcinomas

International Journal of Cancer. Journal International Du Cancer
K KimM J Murnane

Abstract

Point mutation and overexpression are recognized mechanisms for ras activation in malignancy. However, little information is available on overexpression of N-ras protein compared with H- or K-ras proteins, as N-ras-specific antibodies have only recently become available. For comparative analyses of ras protein levels, we have probed Western blots of extracts from 9 normal human tissues and 55 pairs of colorectal carcinoma and matched control mucosa, using monoclonal antibodies (MAbs) specific for H-, K- or N-ras proteins. On multi-tissue blots, N-ras protein was more highly expressed in colon than in the other human tissues analyzed, suggesting a role for N-ras in colorectal function. N-ras protein levels in multiple independent extracts of normal colon mucosa were consistently higher than either K-ras protein or H-ras protein levels. In 69% of colon carcinomas, N-ras protein levels were increased an average of 4.8-fold over normal mucosa. Overexpression of K-ras protein was also observed in colon cancers but less frequently (13% of cases) than N-ras protein. H-ras protein levels were too low for comparative studies. Alterations in N-ras protein mobility, possibly reflecting increased post-translational processing, were also de...Continue Reading

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Citations

Jun 24, 2008·Toxicological Sciences : an Official Journal of the Society of Toxicology·Lanlan ZhouQiaoyuan Yang
Dec 4, 2004·Biological Chemistry·Christopher Jedeszko, Bonnie F Sloane
Jun 22, 1999·Human Pathology·N J MulliganM J O'Brien
Feb 14, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jeongmin SeoRobert S Chapkin
Apr 16, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·David W L MaRobert S Chapkin
Aug 12, 1998·International Journal of Cancer. Journal International Du Cancer·K KimM J Murnane

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