PMID: 8959760Jan 1, 1996Paper

N-terminal 10- and 12-kDa POMC fragments stimulate differentiation of lactotrophs

Peptides
A Van BaelC Denef

Abstract

Medium conditioned by a highly enriched population of gonadotrophs, cultured as reaggregates in the presence of 0.01 nM GnRH, was concentrated, separated on a reversed-phase HPLC column, and tested for activity on lactotroph development in pituitary reaggregate cell cultures of 14-day-old rats. The number of cells expressing prolactin (PRL) mRNA was estimated by image analysis after in situ hybridization of paraffin-embedded sections. The number of these cells entering the mitotic cycle was estimated by autoradiography of [3H]thymidine ([3H]T) incorporation. One HPLC column fraction expanded the section area occupied by PRL mRNA cells without displaying an effect on [3H]T labeling of these cells, indicating that this fraction induces differentiation in the lactotroph lineage. The latter fraction was further purified on a second reversed-phase HPLC column, a gel filtration column, and a final reversed-phase HPLC column. From the last column, four substances were isolated that all selectively induced differentiation of lactotrophs. Each of them had an N-terminal amino acid sequence identical to the N-terminal domain of rat proopiomelanocortin (POMC). As determined by mass spectrometric analysis, the M(r)s were 10,091, 10,289, 12,...Continue Reading

References

May 22, 1992·Science·W E HolmesG D Lewis
Nov 25, 1985·FEBS Letters·J DrouinM Nemer
Nov 1, 1986·Journal of Steroid Biochemistry·M A Seger, H P Bennett
Jul 1, 1994·Proceedings of the Society for Experimental Biology and Medicine·N Ben-Baruch, Y Yarden
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·M MarcinkiewiczM Chrétien
Feb 1, 1996·Journal of Neuroendocrinology·A Van Bael, C Denef

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Citations

Nov 15, 1997·Microscopy Research and Technique·H Vankelecom, C Denef
Oct 23, 1998·Molecular and Cellular Endocrinology·L R BerghmanF Vandesande
Jul 20, 1999·Molecular and Cellular Endocrinology·E SeuntjensC Denef
Nov 25, 1998·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·C Denef, A Van Bael
Apr 26, 2003·Trends in Endocrinology and Metabolism : TEM·Carl Denef
Mar 10, 2001·Clinical and Experimental Pharmacology & Physiology·C DenefA Van Bael
Jul 10, 2003·Annals of the New York Academy of Sciences·C DenefE Swinnen

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