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N-terminal labeling of proteins using initiator tRNA

Methods : a Companion to Methods in Enzymology

Jul 1, 2005

Jerzy OlejnikKenneth J Rothschild

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Abstract

Methodology based on tRNA mediated protein engineering is described for the introduction of fluorophores and other labels at the N-terminus of proteins produced in cell-free translation systems. One method for low-level (trace) N-terminal labeling is based on the use of an Escherichia c...read more

Mentioned in this Paper

SPINK1 gene
Sodium Chloride
DNA Sequence
Amino Acid [EPC]
Crotalus adamanteus
Methionine
Lysine
Centrifugation
Chemicals
Mutant
24
Paper Details
References
  • References21
  • Citations14
12

N-terminal labeling of proteins using initiator tRNA

Methods : a Companion to Methods in Enzymology

Jul 1, 2005

Jerzy OlejnikKenneth J Rothschild

PMID: 16076451

DOI: 10.1016/j.ymeth.2005.04.003

Abstract

Methodology based on tRNA mediated protein engineering is described for the introduction of fluorophores and other labels at the N-terminus of proteins produced in cell-free translation systems. One method for low-level (trace) N-terminal labeling is based on the use of an Escherichia c...read more

Mentioned in this Paper

SPINK1 gene
Sodium Chloride
DNA Sequence
Amino Acid [EPC]
Crotalus adamanteus
24

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Paper Details
References
  • References21
  • Citations14
12

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