N-Terminal Polypeptide of Annexin A2 Decreases Infection of Mycoplasma hyorhinis to Gastric Cancer Cells

PloS One
Shiqin YuanCheng-chao Shou

Abstract

Mycoplasma infection in human and its contamination in cell cultures are worldwide problems. The drugs currently available for preventing or treating mycoplasma infection suffer from low sensitivity, strong resistance and high toxicity. Our previous work showed that Mycoplasma hyorhinis (M. hyorhinis) infection was mediated by the interaction between p37 of M. hyorhinis and Annexin A2 (ANXA2) of host cells, however the translational value of this mechanism was unknown. Herein, we synthesized the N-terminal of ANXA2 polypeptide (A2PP) and found that A2PP could decrease the infection of M. hyorhinis to gastric cancer cells and block M. hyorhinis infection-induced cell migration. Furthermore, we found that A2PP could reduce M. hyorhinis contamination of passage cells. Moreover, compared with the commercial antibiotics commonly used in cell culture to prevent M. hyorhinis infection, A2PP demonstrated a more effectiveness but a low toxicity on cell growth. Thus, our study for the first time revealed A2PP's potential for the treatment and prevention of M. hyorhinis infection.

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Citations

Apr 11, 2018·International Archives of Allergy and Immunology·Yang PengDe-Yun Wang
Dec 22, 2016·Biological Chemistry·Maria-Pilar FernandezReginald O Morgan
Jul 2, 2016·Biological Chemistry·Alexander KuehnlUrsula Rescher
Jun 20, 2018·International Journal of Molecular Sciences·Sebastian SchloerUrsula Rescher
Feb 8, 2021·Journal of Cancer Research and Clinical Oncology·Yueyu DaiWeiguo Hu

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Methods Mentioned

BETA
PCR
electrophoresis
ELISA
Assay
Pull-Down
co-immunoprecipitation assay
Co-immunoprecipitation

Software Mentioned

SPSS
Affymetrix GeneChip Command Console
Genespring

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