PMID: 11341973May 9, 2001Paper

N1E-115 mouse neuroblastoma cells express MT1 melatonin receptors and produce neurites in response to melatonin

Biochimica Et Biophysica Acta
S L BordtM A Melan

Abstract

Melatonin, a pineal hormone that induces sleep, has become a popular over-the-counter drug. The cellular effects of melatonin, however, are only beginning to be studied. We have recently shown that stimulation of the MT1 melatonin receptor induces rapid and dramatic cytoskeletal rearrangements in transformed non-neuronal cells (Witt-Enderby et al., Cell. Motil. Cytoskel. 46 (2000) 28). These cytoskeletal changes result in the formation of structures that closely resemble neurites. In this work, we show that the N1E-115 mouse neuroblastoma cell line rapidly responds to melatonin stimulation and forms neurites within 24 h. We also demonstrate that these cells readily bind 2-[125I]iodomelatonin at levels consistent with what is noted for native tissues (B(max)=3.43+/-1.56 fmol/mg protein; K(d)=240 pM). Western analysis shows that these cells possess and express melatonin receptors of the MT1 subtype. Treatment with pertussis toxin eliminates neurite formation whereas treatment with the MT2 subtype-specific activator, BMNEP, does not induce neurite formation. We have previously shown that increases in MEK 1/2 and ERK 1/2 phosphorylation are correlated with the shape changes in transformed CHO cells. Western analysis of the MEK/ERK ...Continue Reading

References

Jan 1, 1990·Journal of Pineal Research·G Benítez-KingF Antón-Tay
Feb 1, 1995·Trends in Pharmacological Sciences·M L Dubocovich
Jan 1, 1993·Life Sciences·G Benítez-KingF Antón-Tay
Oct 1, 1995·Journal of Pineal Research·E J CostaM T Lamy-Freund
Jan 16, 1997·The New England Journal of Medicine·A Brzezinski
Jan 22, 1998·Journal of Medicinal Chemistry·P A Witt-EnderbyP K Li

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Citations

May 29, 2004·Journal of Physiology and Biochemistry·P MontillaI Túnez
Mar 12, 2003·Life Sciences·Paula A Witt-EnderbyMelissa A Melan
Dec 21, 2005·Acta Pharmacologica Sinica·Jian-zhi Wang, Ze-fen Wang
Dec 28, 2011·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Gregory A CaryAndrea R Tilden
Jul 31, 2003·Journal of Pineal Research·Yoichi KobayashiBunpei Ishizuka
Oct 4, 2006·Journal of Pineal Research·Michael J JarzynkaPaula A Witt-Enderby
Jun 26, 2012·Journal of Pineal Research·José L MaurizJavier González-Gallego
Nov 24, 2011·Journal of Pineal Research·Sergio A Rosales-CorralRussel J Reiter
Nov 30, 2005·Journal of Pineal Research·Gloria Benítez-King
Mar 5, 2014·The Journal of Steroid Biochemistry and Molecular Biology·Nootchanart RukseePiyarat Govitrapong
Apr 4, 2002·Neuroreport·Kristen J Armstrong, Lennard P Niles
May 14, 2017·Journal of Pineal Research·Tania Galván-ArrietaGloria Benítez-King
May 29, 2013·International Journal of Molecular Sciences·Di Yan LiQing Zhu
Nov 16, 2018·The International Journal of Neuroscience·Bita BeigiNasrin Hashemi-Firouzi
Feb 9, 2010·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Lucia de Fatima Sobral Sampaio, Regina Pekelmann Markus
Mar 4, 2017·Inflammopharmacology·M NajafiS Rezapoor

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