PMID: 11607653Apr 16, 1996Paper

N2 fixation in marine heterotrophic bacteria: dynamics of environmental and molecular regulation

Proceedings of the National Academy of Sciences of the United States of America
J A CoyerR S Alberte

Abstract

Molecular and immunological techniques were used to examine N2 fixation in a ubiquitous heterotrophic marine bacterium, the facultative anaerobic Vibrio natriegens. When batch cultures were shifted from aerobic N-replete to anaerobic N-deplete conditions, transcriptional and post-translational regulation of N2 fixation was observed. Levels of nifHDK mRNA encoding the nitrogenase enzyme were highest at 140 min postshift and undetectable between 6 and 9 h later. Immunologically determined levels of nitrogenase enzyme (Fe protein) were highest between 6 and 15 h postshift, and nitrogenase activity peaked between 6 and 9 h postshift, declining by a factor of 2 after 12-15 h. Unlike their regulation in cyanobacteria, Fe protein and nitrogenase activity were present when nifHDK mRNA was absent in V. natriegens, indicating that nitrogenase is stored and stable under anaerobic conditions. Both nifHDK mRNA and Fe protein disappeared within 40 min after cultures were shifted from N2-fixing conditions (anaerobic, N-deplete) to non- N2-fixing conditions (aerobic, N-enriched) but reappeared when shifted to conditions favoring N2 fixation. Thus, unlike other N2-fixing heterotrophic bacteria, nitrogenase must be resynthesized after aerobic ex...Continue Reading

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Citations

Jun 15, 2010·SAR and QSAR in Environmental Research·P M SivakumarM Doble
Jun 9, 2016·Journal of Plankton Research·Shunyan CheungHongbin Liu
Sep 10, 2005·Applied and Environmental Microbiology·Matthew J ChurchJonathan P Zehr
Jan 5, 2005·Journal of Bacteriology·Kazuhisa OkadaTakeshi Honda
Jun 28, 2019·Scientific Reports·Eyal GeislerEdo Bar-Zeev

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