N7-Methylguanine at position 46 (m7G46) in tRNA from Thermus thermophilus is required for cell viability at high temperatures through a tRNA modification network.

Nucleic Acids Research
Chie TomikawaHiroyuki Hori

Abstract

N(7)-methylguanine at position 46 (m(7)G46) in tRNA is produced by tRNA (m(7)G46) methyltransferase (TrmB). To clarify the role of this modification, we made a trmB gene disruptant (DeltatrmB) of Thermus thermophilus, an extreme thermophilic eubacterium. The absence of TrmB activity in cell extract from the DeltatrmB strain and the lack of the m(7)G46 modification in tRNA(Phe) were confirmed by enzyme assay, nucleoside analysis and RNA sequencing. When the DeltatrmB strain was cultured at high temperatures, several modified nucleotides in tRNA were hypo-modified in addition to the lack of the m(7)G46 modification. Assays with tRNA modification enzymes revealed hypo-modifications of Gm18 and m(1)G37, suggesting that the m(7)G46 positively affects their formations. Although the lack of the m(7)G46 modification and the hypo-modifications do not affect the Phe charging activity of tRNA(Phe), they cause a decrease in melting temperature of class I tRNA and degradation of tRNA(Phe) and tRNA(Ile). (35)S-Met incorporation into proteins revealed that protein synthesis in DeltatrmB cells is depressed above 70 degrees C. At 80 degrees C, the DeltatrmB strain exhibits a severe growth defect. Thus, the m(7)G46 modification is required for c...Continue Reading

References

Aug 10, 1979·Nucleic Acids Research·Y KuchinoS Nishimura
Mar 1, 1978·Nucleic Acids Research·U Pachmann, H G Zachau
Oct 4, 1976·Biochemical and Biophysical Research Communications·K WatanabeS Nishimura
May 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·B C PerssonG R Björk
Aug 16, 1974·Nature·J D RobertusA Klug
Dec 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·S H KimA RICH
Feb 21, 1998·Nucleic Acids Research·M SprinzlS Steinberg
Sep 25, 1998·The Journal of Biological Chemistry·H HoriK Watanabe
Dec 10, 1998·Nucleic Acids Research·J RozenskiJ A McCloskey
Mar 17, 1999·The EMBO Journal·P J Farabaugh, G R Björk
Apr 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·J AndersonA G Hinnebusch
Nov 7, 2000·Progress in Biophysics and Molecular Biology·S YokoyamaS Kuramitsu
Dec 5, 2000·Nature Structural Biology·S YokoyamaS Kuramitsu
Mar 29, 2002·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Hiroyuki HoriKimitsuna Watanabe
Jul 12, 2002·The EMBO Journal·Jeannette WolfWalter Keller
May 6, 2003·Journal of Bacteriology·Lara G S De BieJanusz M Bujnicki
Sep 11, 2004·The Journal of Biological Chemistry·Hironori OkamotoHiroyuki Hori
Jan 8, 2005·The Journal of Biological Chemistry·Kazunori WatanabeHiroyuki Hori
Dec 1, 2005·The Journal of Biological Chemistry·Naoki ShigiKimitsuna Watanabe
Dec 31, 2005·Nucleic Acids Research·Stanislaw Dunin-HorkawiczJanusz M Bujnicki
Jan 3, 2006·Molecular Cell·Andrei AlexandrovEric M Phizicky
Mar 21, 2006·The Journal of Biological Chemistry·Naoki ShigiKimitsuna Watanabe
Apr 8, 2006·Nucleic Acids Research·Ingrid ZegersLouis Droogmans
Sep 12, 2006·The Journal of Biological Chemistry·Kazunori WatanabeHiroyuki Hori
Nov 24, 2006·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Hiroshi TakedaHiroyuki Hori

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Citations

Oct 26, 2012·The Journal of Biological Chemistry·Ryota YamagamiHiroyuki Hori
Aug 22, 2012·Annual Review of Genetics·Basma El YacoubiValérie de Crécy-Lagard
Mar 19, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Susan P Russell, Patrick A Limbach
Dec 30, 2014·International Journal of Molecular Sciences·Takuya KawamuraHiroyuki Hori
Aug 9, 2011·Wiley Interdisciplinary Reviews. RNA·Yuri Motorin, Mark Helm
Nov 29, 2013·Mass Spectrometry Reviews·Ed Dudley, Liz Bond
Jan 28, 2015·RNA Biology·Michael P Guy, Eric M Phizicky
Jun 7, 2014·Frontiers in Genetics·Hiroyuki Hori
May 31, 2016·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Ryota YamagamiHiroyuki Hori
Feb 24, 2017·Biomolecules·Stephanie OerumCarine Tisné
Mar 12, 2017·Biomolecules·Richard J Maraia, Aneeshkumar G Arimbasseri
Apr 5, 2017·Biomolecules·Christian LorenzMario Mörl
May 26, 2017·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Misa NakashimaHiroyuki Hori
Dec 13, 2018·Nucleic Acids Research·Jing LiRu-Juan Liu
Jan 10, 2019·Proceedings of the National Academy of Sciences of the United States of America·Satoshi Kimura, Matthew K Waldor
Dec 12, 2019·The Analyst·Priti ThakurBalasubrahmanyam Addepalli
Jan 17, 2015·The Journal of Biological Chemistry·Hiroyuki TakumaHiroyuki Hori
Nov 2, 2017·Journal of Biochemistry·Chie TomikawaSatoko Yoshizawa
Sep 20, 2019·Neural Regeneration Research·Sherif RashadTeiji Tominaga
Nov 20, 2019·Nature Chemical Biology·Takashi YokogawaSatoshi Ohno
Dec 20, 2018·International Journal of Molecular Sciences·Chie Tomikawa

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Methods Mentioned

BETA
column chromatography
PCR
electrophoresis
aminoacylation
aminoacylations
gel-filtration

Software Mentioned

BLAST
search

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