Abstract
While there is considerable evidence for phosphate (Pi) reabsorption in the distal tubule, Pi transport and its regulation have not been well characterized in this segment of the nephron. In the present study, we examined Na+-dependent Pi transport in immortalized mouse distal convoluted tubule (MDCT) cells. Pi uptake by MDCT cells is Na+-dependent and, under initial rate conditions, is inhibited by phosphonoformic acid (41 +/- 3% of control), a competitive inhibitor of Na+-Pi cotransport. The transport system has a high affinity for Pi (Km = 0.46 mM) and is stimulated by lowering the extracellular pH from 7.4 to 6.4 and inhibited by raising the pH from 7.4 to 8.4. Exposure to Pi-free medium for 21 h increased Na+-Pi cotransport from 2.1 to 5.5 nmol/mg of protein/5 minutes (p < 0.05) while parathyroid hormone, forskolin, and phorbol 12-myristate 13-acetate failed to alter Pi uptake in MDCT cells. Reverse transcriptase polymerase chain reaction of MDCT cell RNA provided evidence for the expression of the Npt1 but not the Npt2 Na+-Pi cotransporter gene. However, preincubation of MDCT cells with Npt1 antisense oligonucleotide led to only 20% inhibition of Na+-Pi cotransport, suggesting that other Na+-Pi cotransporters are operativ...Continue Reading
References
Jun 8, 1977·Pflügers Archiv : European journal of physiology·R GregerF G Knox
Aug 1, 1992·The Journal of Clinical Investigation·F A Gesek, P A Friedman
Sep 1, 1992·The Journal of Clinical Investigation·F A Gesek, P A Friedman
May 1, 1991·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·J H PizzoniaP A Friedman
Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·A WernerH Murer
Aug 1, 1990·Pflügers Archiv : European journal of physiology·S J ReshkinH Murer
Feb 1, 1989·The American Journal of Physiology·B EscoubetC Amiel
Jul 1, 1986·The American Journal of Physiology·K Malmström, H Murer
Feb 1, 1988·The Journal of Clinical Endocrinology and Metabolism·L SartoriK L Insogna
Sep 1, 1972·The Journal of Clinical Investigation·B B StaumM Goldberg
Sep 1, 1995·Pflügers Archiv : European journal of physiology·C M HartmannH Murer
Mar 15, 1995·European Journal of Biochemistry·C HelpsJ McGivan
Jul 19, 1994·Proceedings of the National Academy of Sciences of the United States of America·M P KavanaughA D Miller
Aug 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J F Collins, F K Ghishan
Nov 1, 1993·Genomics·S S ChongM R Hughes
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·S MagagninH Murer
Jul 1, 1993·The American Journal of Physiology·B EscoubetC Amiel
Jul 1, 1993·The American Journal of Physiology·H S Tenenhouse, J Martel
Aug 1, 1993·Pflügers Archiv : European journal of physiology·M CusterH Murer
Aug 1, 1993·Pflügers Archiv : European journal of physiology·J BiberH Murer
Jan 1, 1996·Endocrinology·P A FriedmanF A Gesek
Nov 1, 1995·Kidney International·P A Friedman, F A Gesek
Feb 16, 1996·The Journal of Biological Chemistry·S Saxena, M Allon
Jan 1, 1996·Annual Review of Physiology·H Murer, J Biber
Feb 1, 1997·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·H S Tenenhouse
Mar 15, 1997·The Journal of Clinical Investigation·L BeckH S Tenenhouse
Citations
Aug 31, 2001·Microbiology and Molecular Biology Reviews : MMBR·J OverbaughM V Eiden
Apr 18, 2007·Molecular and Cellular Biology·Yuji YoshikoJane E Aubin
Aug 14, 2009·Kidney International·Glen S Markowitz, Mark A Perazella
Aug 4, 2012·PloS One·Maria HallerRainer Oberbauer
Jun 19, 2001·The Journal of Biological Chemistry·D WangJ E Henderson
Jun 1, 2000·Endocrinology·N Zhao, H S Tenenhouse
Apr 21, 2012·Clinical Journal of the American Society of Nephrology : CJASN·Eleanor Lederer, Ken-ichi Miyamoto
May 15, 2007·American Journal of Physiology. Cell Physiology·Silvia RaveraIan C Forster
Sep 24, 1999·Current Opinion in Nephrology and Hypertension·H S Tenenhouse
Jun 22, 2007·American Journal of Physiology. Renal Physiology·Leila V VirkkiIan C Forster
Nov 13, 2001·American Journal of Physiology. Renal Physiology·Y SoumounouH S Tenenhouse
Jul 28, 2016·Australian Veterinary Journal·J B King
Oct 1, 1998·The American Journal of Physiology·H S TenenhouseC Gauthier
Apr 13, 2004·American Journal of Physiology. Renal Physiology·Brian E PeerceRebecca D Clarke
May 1, 2020·Physiological Reviews·Nati HernandoEleanor Lederer
Oct 4, 2000·Physiological Reviews·H MurerJ Biber
Sep 4, 2003·American Journal of Physiology. Renal Physiology·Harriet S TenenhouseKen-ichi Miyamoto
Feb 13, 2021·Viruses·Christopher Perry, Andrea C M E Rayat
Oct 12, 1999·The American Journal of Physiology·I FernandesC Silve