Na+ absorption by Claudius' cells is regulated by purinergic signaling in the cochlea

Acta Oto-laryngologica
Jae Chul YooJun Ho Lee

Abstract

Claudius' cells absorb Na(+) through the amiloride-sensitive epithelial sodium channel (ENaC). Transepithelial ion transport through ENaC and possibly a Cl(-) secretory pathway is regulated by P2Y purinergic signaling. The purpose of this study was to investigate ion transport in Claudius' cells and its purinergic regulation. Young adult Sprague-Dawley rats and gerbils were studied. The Claudius' cell layer on the basilar membrane was dissected from the basal turn of the cochlea. A voltage-sensitive vibrating probe was used to measure transepithelial short circuit current (I(sc) ). The baseline I(sc) of Claudius' cells was measured in the perilymph-like control solution and the change of I(sc) after application of amiloride (10 μM) or uridine triphosphate (UTP, 100 μM). A negative baseline I(sc) was observed in the control solution (-12.50 ± 3.95 μA/cm(2), n = 8) and the addition of amiloride resulted in a decrease of I(sc) by 75.8%. The application of UTP, an agonist for P2Y purinergic receptors, led to a partial inhibition of I(sc) (by 38.2 ± 3.2%, n = 5), and subsequent addition of amiloride abolished the remaining I(sc).

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Citations

Oct 13, 2012·Auris, Nasus, Larynx·Chang-Hee KimJun Ho Lee
Jun 30, 2018·Physiological Genomics·Janet L Fitzakerley, George J Trachte

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