PMID: 8584404Nov 1, 1995Paper

Na+ and Cl- conductances in airway epithelial cells: increased Na+ conductance in cystic fibrosis

Pflügers Archiv : European journal of physiology
Karl KunzelmannR Greger

Abstract

Na+ and Cl- conductances in the apical membrane of respiratory epithelial cells are essential for electrolyte and water transport in the airways. Apart from the well described defect in adenosine 3' : 5' cyclic monophosphate-(cAMP-) dependent activation of Cl- conductances in cystic fibrosis (CF), an increased Na+ conductance has also been reported from transepithelial measurements. In the present experiments we tried to identify these conductances in nasal epithelial cells using patch-clamp and microelectrode techniques. With these methods we found identical and relatively low membrane voltages of about -36 mV in both freshly isolated and primary cultured normal and CF nasal epithelial cells. A Cl- conductance could be activated by cAMP in normal (deltaG = 0.3 +/- 0.8 nS, n = 10) but not in CF (deltaG = 0.3 +/- 0.1 nS, n = 11) cells, whereas Ca2+-dependent Cl- currents activated by adenosine 5'-triphosphate (ATP) and bradykinin were present in both types of cells. Cell-attached membrane patches from stimulated cells did not reveal discernible single-channel events when activated with any of the agonists. A Na+ conductance was also detected in freshly isolated ciliated respiratory cells in impalement studies, as evidenced by th...Continue Reading

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Citations

Feb 1, 1996·Pflügers Archiv : European journal of physiology·K KunzelmannR Gregner
Jan 1, 1996·Pflügers Archiv : European journal of physiology·D EckeR Greger
Aug 1, 1996·Pflügers Archiv : European journal of physiology·R Greger
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