Na+ competes with K+ in bumetanide-sensitive transport by Malpighian tubules of Rhodnius prolixus

The Journal of Experimental Biology
Juan P IanowskiMichael J O'Donnell

Abstract

We examined the effects of bathing saline Na+/K+ ratio, bumetanide and hydrochlorothiazide on fluid and ion transport by serotonin-stimulated Malpighian tubules of Rhodnius prolixus. Previous pharmacological and electrophysiological studies indicate that a bumetanide-sensitive Na+/K+/2Cl- cotransporter is the primary route for basolateral ion entry into the cell during fluid secretion. The goal of this study was to resolve the apparent conflict between relatively high secretion rates by tubules bathed in K+-free saline and the evidence that Na+/K+/2Cl- cotransporters described in other systems have an absolute requirement for all three ions for translocation. Our measurements of fluid secretion rate, ion fluxes and electrophysiological responses to serotonin show that fluid secretion in K+-free saline is bumetanide sensitive and hydrochlorothiazide insensitive. Dose-response curves of secretion rate versus bumetanide concentration were identical for tubules bathed in K+-free and control saline with IC50 values of 2.6 x 10(-6) mmol l(-1) and 2.9 x 10(-6) mmol l(-1), respectively. Double-reciprocal plots of K+ flux versus bathing saline K+ concentration showed that increasing Na+ concentration in the bathing fluid increased Kt bu...Continue Reading

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Citations

Oct 30, 2008·The Journal of Biological Chemistry·Eva V WertheimerPablo E Visconti
Aug 24, 2012·American Journal of Physiology. Cell Physiology·Aylin R RodanChou-Long Huang
Jul 21, 2007·Journal of Insect Physiology·Esau Ruiz-SanchezMichael J O'Donnell
Apr 19, 2015·Integrative and Comparative Biology·Dirk Weihrauch, Michael J O'Donnell
Jan 11, 2014·Journal of Insect Physiology·Evan K Pacey, Michael J O'Donnell
May 3, 2019·The Journal of Experimental Biology·Klaus W Beyenbach
May 6, 2019·The Journal of Experimental Biology·Natalia CapriottiSheila Ons
Jul 11, 2014·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Paula GioinoJuan P Ianowski
Jul 25, 2014·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Matthew F RouhierKlaus W Beyenbach
Dec 9, 2017·The Journal of Experimental Biology·Natalie M D'Silva, Michael J O'Donnell
Dec 17, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Juan P IanowskiIan Orchard
Mar 5, 2020·American Journal of Physiology. Lung Cellular and Molecular Physiology·Xiaojie LuanJuan P Ianowski
Jan 20, 2009·The Journal of Experimental Biology·M J O'Donnell

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