Na+-dependent activation of NADH oxidase in membrane fractions from halophilic Vibrio alginolyticus and V. costicolus

Journal of Biochemistry
T UnemotoM Hayashi

Abstract

Membrane-bound NADH oxidase activities from slightly halophilic marine Vibrio alginolyticus and moderately halophilic V. costicolus required 0.3 and 0.5 M Na+, respectively, for maximum activity; other cations such as Li+,K+,Rb+,Cs+,Mg2+, and Ca2+ were relatively ineffective as replacements for Na+. The concentration of Na+ required to give half-maximum activity with the NADH oxidase from V. alginolyticus was 82 mN. This value was reduced to 6.4 and 13.8 mM in the presence of 400 mM K+ and 10 mM Mg2+, respectively, indicating that K+ and Mg2+ cooperated with Na+ for activation. The same pattern of cation dependence was observed with the NADH oxidase from V. costicolus. The NADH oxidase from nonhalophilic Escherichia coli, however, had no specific requirement for Na+. Thus, Na+-dependent activation of NADH oxidase appeared to be a characteristic feature of these halophilic bacteria. All NADH oxidases examined were influenced by the species of anion present and the order of activating effect followed the lyotropic series:SO4(2-), CH3COO- greater than Cl- greater than NO3- greater than SCN-. Chaotropic anions such as NO3- and SCN- were inhibitory to the NADH oxidases, irrespective of the halophilic nature of the bacterial source.

Citations

Jan 1, 1988·Critical Reviews in Microbiology·T A KrulwichA A Guffanti
Apr 6, 2005·Biochemistry. Biokhimii︠a︡·Alexander V Bogachev, M I Verkhovsky
Dec 1, 1989·Journal of Bioenergetics and Biomembranes·T Unemoto, M Hayashi
Sep 30, 2017·FEMS Microbiology Reviews·Pavel DibrovGrant N Pierce
Jun 19, 1989·FEBS Letters·V P Skulachev
Aug 1, 1993·Journal of Bioenergetics and Biomembranes·T Unemoto, M Hayashi
Jan 1, 2015·Applied Microbiology and Biotechnology·Ravindra Pal SinghC R K Reddy
Dec 1, 1989·Journal of Bioenergetics and Biomembranes·H Tokuda

Related Concepts

Cations, Monovalent
Plasma Membrane
Cell Membrane Permeability
Enzyme Activation
NADH, NADPH Oxidoreductases
Sodium
Beneckea

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