PMID: 8944653Nov 1, 1996Paper

Na-K-ATPase in lacrimal gland acinar cell endosomal system: correcting a case of mistaken identity

The American Journal of Physiology
J P GierowA K Mircheff

Abstract

Na-K-ATPase is associated with a variety of membrane populations in lacrimal acinar cells. Acinus-like structures formed by rabbit acinar cells in primary culture were incubated with horseradish peroxidase (HRP) to label basolateral and endosomal membranes and then analyzed by electron microscopy cytochemistry with the 3-3'-diaminobenzidine reaction or by fractionation and measurement of marker catalytic activities or immunoreactivities. HRP adsorbed to basolateral membranes at 4 degrees C. Fractionation showed it associated with low-density membranes enriched in acid phosphatase and TGN38 but containing only minor amounts of Na-K-ATPase. Cells internalized HRP to cytoplasmic vesicles, Golgi structures, and lysosomes at 37 degrees C. The major endosomal compartment revealed by fractionation coincided with major peaks of Na-K-ATPase and Rab6 and secondary peaks of galactosyltransferase and gamma-adaptin. Carbachol (10 microM) increased lysosomal and Golgi labeling. Thus most of the Na-K-ATPase is located in the basolateral membrane-oriented endosomal system, concentrated in a compartment possibly related to the trans-Golgi network. Constitutive and stimulation-accelerated traffic to and from this compartment may serve several ex...Continue Reading

References

Jan 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R FuchsI Mellman
Jan 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·C C CainR F Murphy
Jan 1, 1985·The Journal of Membrane Biology·A K MircheffG M Gray
May 1, 1995·Experimental Eye Research·J P GierowA K Mircheff

❮ Previous
Next ❯

Citations

Dec 14, 2006·American Journal of Physiology. Endocrinology and Metabolism·Yanru WangAustin K Mircheff
Apr 29, 2011·American Journal of Physiology. Cell Physiology·Lilian ChiangSarah F Hamm-Alvarez
Dec 6, 2011·Cytotechnology·Janette ContrerasSarah F Hamm-Alvarez
Sep 27, 2012·Current Eye Research·Jianyan HuangChuanqing Ding
May 15, 2010·Current Eye Research·Stina K CarlssonJ Peter Gierow
Aug 9, 2002·DNA and Cell Biology·Austin K MircheffSarah F Hamm-Alvarez
Dec 9, 1998·The American Journal of Physiology·M SoneeS F Hamm-Alvarez
Nov 24, 1999·The American Journal of Physiology·T YangA K Mircheff
Mar 7, 2002·Experimental Eye Research·Limin QianAustin K Mircheff
Aug 15, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·L EkbladJ P Gierow
Apr 25, 2006·Experimental Eye Research·Sofia V AnderssonJ Peter Gierow
Jul 15, 2006·Experimental Eye Research·Sofia V AnderssonJ Peter Gierow
Oct 2, 2007·Experimental Eye Research·Yanru WangJoel E Schechter

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.