Na(+)-K (+) pump location and translocation during muscle contraction in rat skeletal muscle.

Pflügers Archiv : European journal of physiology
Michael KristensenCarsten Juel

Abstract

Muscle contraction may up-regulate the number of Na(+)-K(+) pumps in the plasma membrane by translocation of subunits. Since there is still controversy about where this translocation takes place from and if it takes place at all, the present study used different techniques to characterize the translocation. Electrical stimulation and biotin labeling of rat muscle revealed a 40% and 18% increase in the amounts of the Na(+)-K(+) pump alpha(2) subunit and caveolin-3 (Cav-3), respectively, in the sarcolemma. Exercise induced a 36% and 19% increase in the relative amounts of the alpha(2) subunit and Cav-3, respectively, in an outer-membrane-enriched fraction and a 41% and 17% increase, respectively, in sarcolemma giant vesicles. The Na(+)-K(+) pump activity measured with the 3-O-MFPase assay was increased by 37% in giant vesicles from exercised rats. Immunoprecipitation with Cav-3 antibody showed that 17%, 11% and 14% of the alpha(1) subunits were associated with Cav-3 in soleus, extensor digitorum longus, and mixed muscles, respectively. For the alpha(2), the corresponding values were 17%, 5% and 16%. In conclusion; muscle contraction induces translocation of the alpha subunits, which is suggested to be caused partly by structural ...Continue Reading

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Citations

Feb 24, 2010·The Journal of Membrane Biology·Michael Kristensen, Carsten Juel
Jun 21, 2011·The Journal of Membrane Biology·Maria S IngwersenCarsten Juel
Sep 2, 2008·Annual Review of Physiology·Jang H Youn, Alicia A McDonough
Jan 13, 2016·The Journal of General Physiology·Violetta V KravtsovaIgor I Krivoi
Sep 16, 2015·The Journal of General Physiology·Marino DiFrancoJudith A Heiny
Feb 9, 2012·Biochemical and Biophysical Research Communications·Yasuro FuruichiKazumi Masuda
Feb 6, 2015·BioMed Research International·Violetta V KravtsovaIgor I Krivoi
Jun 3, 2016·Frontiers in Physiology·Vladimir V Matchkov, Igor I Krivoi
May 12, 2016·American Journal of Physiology. Endocrinology and Metabolism·Sergej Pirkmajer, Alexander V Chibalin
Jun 19, 2012·Journal of Applied Physiology·Martin Broch-LipsCraig A Goodman
Nov 7, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Carsten Juel
Jan 6, 2021·Journal of Muscle Research and Cell Motility·Sergej PirkmajerAlexander V Chibalin

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