PMID: 8611467Apr 1, 1996Paper

NA-phenotype-dependent differences in neutrophil Fc gamma RIIIb expression cause differences in plasma levels of soluble Fc gamma RIII

British Journal of Haematology
H R KoeneA E von dem Borne

Abstract

Soluble Fc gamma RIII in plasma is primarily derived from neutrophils and is a measure of the total body neutrophil mass. We have developed a new, sensitive 'sandwich' ELISA to measure soluble Fc gamma RIII in plasma and released Fc gamma RIII in cell supernatants. Both sFc gamma RIIIa, derived from NK cells and sFc gamma RIIIb, derived from neutrophils are detected in the assay. However, plasma analysis of Fc gamma RIIIB gene-deficient donors suggested that sFc gamma RIIIa contributes only marginally to the total amount measured in healthy individuals. Furthermore, we observed that plasma of homozygous NA1-positive donors contained lower amounts of sFc gamma RIII than plasma of homozygous NA2-positive donors. Heterozygous donors were found to have intermediate levels of sFc gamma RIII in their plasma. Hemizygous Fc gamma RIIIB gene-deficient donors were found to have half the amount of sFc gamma RIII in their plasma compared to donors with two Fc gamma RIIIB alleles. These NA phenotype-dependent differences in plasma sFc gamma RIII could not be contributed to either an assay artefact or NA-dependent differences in shedding of Fc gamma RIIIb upon neutrophil activation. Calibration curves constructed with plasma of homozygous do...Continue Reading

Citations

Mar 10, 2001·Transfusion·A RizkR Weinstein
Sep 14, 2011·Current Opinion in Hematology·Stefan MuschterAndreas Greinacher
Jul 27, 2001·Clinical and Experimental Immunology·P J MiddelhovenD Roos
May 7, 2010·Genes and Immunity·M GrothM Platzer
Jan 23, 2008·Vox Sanguinis·J Bux
Jan 23, 2004·Genes and Immunity·V MagnussonUNKNOWN SLE Genetics Collaboration Group
Jan 15, 2009·Human Mutation·Edward J HolloxTushna Dehnugara
Sep 26, 1997·FEBS Letters·P J MiddelhovenA J Verhoeven
Nov 20, 1998·Clinical and Experimental Immunology·A C Muller KoboldJ W Tervaert
Jan 1, 1997·International Reviews of Immunology·J GalonC Sautès
Mar 2, 1999·Biochemical and Biophysical Research Communications·P J MiddelhovenP L Hordijk

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