NA14 is a novel nuclear autoantigen with a coiled-coil domain.

The Journal of Biological Chemistry
F Ramos-MoralesR M Rios

Abstract

The serum from a patient with Sjögren's syndrome (RM serum) was used to screen a human testis cDNA expression library. A cDNA of 865 base pairs containing the entire coding sequence for a novel protein was isolated. The 14-kDa predicted protein contains an acidic domain (amino acids 6-80) with a high frequency of heptad repeats characteristic of alpha-helices that form dimeric coiled-coil structures and an alkaline carboxyl-terminal domain (amino acids 81-119). It seems to be widely expressed, but its expression level varies depending on tissues. A protein of apparent molecular mass of 14 kDa was immunoprecipitated from cell lysates by the autoimmune serum, and it was recognized by rabbit antibodies raised to a recombinant bacterial fusion protein generated from the cDNA clone. Conventional and confocal immunofluorescence microscopy on HeLa and 3T3 cells transiently transfected with a tagged form of the protein showed numerous punctate structures scattered throughout the nucleus. This novel protein has been termed NA14 for Nuclear Autoantigen of 14 kDa.

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Citations

Oct 20, 2011·Protein Engineering, Design & Selection : PEDS·Mar Rodríguez-RodríguezM Angeles Jiménez
Jun 9, 2005·Cell Motility and the Cytoskeleton·Jutta SchoppmeierKarl-Ferdinand Lechtreck
Sep 27, 2018·Nature Cell Biology·Nirakar BasnetNaoko Mizuno

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