Nab3 facilitates the function of the TRAMP complex in RNA processing via recruitment of Rrp6 independent of Nrd1

PLoS Genetics
Milo B FaskenAnita H Corbett

Abstract

Non-coding RNAs (ncRNAs) play critical roles in gene regulation. In eukaryotic cells, ncRNAs are processed and/or degraded by the nuclear exosome, a ribonuclease complex containing catalytic subunits Dis3 and Rrp6. The TRAMP (Trf4/5-Air1/2-Mtr4 polyadenylation) complex is a critical exosome cofactor in budding yeast that stimulates the exosome to process/degrade ncRNAs and human TRAMP components have recently been identified. Importantly, mutations in exosome and exosome cofactor genes cause neurodegenerative disease. How the TRAMP complex interacts with other exosome cofactors to orchestrate regulation of the exosome is an open question. To identify novel interactions of the TRAMP exosome cofactor, we performed a high copy suppressor screen of a thermosensitive air1/2 TRAMP mutant. Here, we report that the Nab3 RNA-binding protein of the Nrd1-Nab3-Sen1 (NNS) complex is a potent suppressor of TRAMP mutants. Unlike Nab3, Nrd1 and Sen1 do not suppress TRAMP mutants and Nrd1 binding is not required for Nab3-mediated suppression of TRAMP suggesting an independent role for Nab3. Critically, Nab3 decreases ncRNA levels in TRAMP mutants, Nab3-mediated suppression of air1/2 cells requires the nuclear exosome component, Rrp6, and Nab3 d...Continue Reading

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Nov 28, 2015·Wiley Interdisciplinary Reviews. RNA·Melanie J Fox, Amber L Mosley
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Datasets Mentioned

BETA
Q9UKM9

Methods Mentioned

BETA
NMR
ChIP
RNA-Seq
PCR
Protein Assay
immunoprecipitation
fluorescence microscopy

Software Mentioned

Adobe
Excel
ClustalW2
Adobe Photoshop
MacPyMOL
hRALY
BLAST
BoxShade
IMD2 CUT
ImageJ

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