NABP1, a novel RORgamma-regulated gene encoding a single-stranded nucleic-acid-binding protein

The Biochemical Journal
Hong Soon KangA M Jetten

Abstract

RORgamma2 (retinoid-related orphan receptor gamma2) plays a critical role in the regulation of thymopoiesis. Microarray analysis was performed in order to uncover differences in gene expression between thymocytes of wild-type and RORgamma-/- mice. This analysis identified a novel gene encoding a 22 kDa protein, referred to as NABP1 (nucleic-acid-binding protein 1). This subsequently led to the identification of an additional protein, closely related to NABP1, designated NABP2. Both proteins contain an OB (oligonucleotide/oligosaccharide binding) motif at their N-terminus. This motif is highly conserved between the two proteins. NABP1 is highly expressed in the thymus of wild-type mice and is greatly suppressed in RORgamma-/- mice. During thymopoiesis, NABP1 mRNA expression is restricted to CD4+CD8+ thymocytes, an expression pattern similar to that observed for RORgamma2. These observations appear to suggest that NABP1 expression is regulated either directly or indirectly by RORgamma2. Confocal microscopic analysis showed that the NABP1 protein localizes to the nucleus. Analysis of nuclear proteins by size-exclusion chromatography indicated that NABP1 is part of a high molecular-mass protein complex. Since the OB-fold is frequen...Continue Reading

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Citations

Jan 30, 2008·Molecular and Cellular Biology·Yong-Sik KimAnton M Jetten
Sep 30, 2009·The Journal of Cell Biology·Jeffrey R SkaarMichele Pagano
Nov 10, 2015·Biochimica Et Biophysica Acta·Venkatasubramanian VidhyasagarYuliang Wu
Apr 19, 2007·Journal of Pineal Research·Elmar PeschkeEckhard Mühlbauer
May 10, 2007·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Ju Youn BeakAnton M Jetten
Apr 4, 2018·Mobile DNA·Alexandra HerrmannThomas Gramberg
Apr 29, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Didier BoucherKum Kum Khanna
Feb 6, 2007·The Journal of Biological Chemistry·Xuexian O YangChen Dong
Dec 16, 2011·Immunological Reviews·Gérard Eberl

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