Na(+)/Ca(2+) exchanger in GABAergic presynaptic boutons of rat central neurons

Journal of Neurophysiology
Atsushi DoiN Akaike

Abstract

Rat Meynert neurons were acutely isolated using a dissociation technique that maintains functional GABAergic presynaptic boutons. Miniature inhibitory postsynaptic currents (mIPSCs) were recorded under voltage-clamp conditions using whole cell patch-clamp recordings. Using the frequency of mIPSCs as a measure of presynaptic terminal excitability, the existence of a Na(+)/Ca(2+) exchanger (NCX) in these GABAergic nerve terminals was clearly demonstrated. Both the frequency and the amplitude of mIPSCs were unaffected by replacement of extracellular Na(2+). However, in this Na(+)-free external solution, ouabain could now induce a transient increase of mIPSCs frequency, which was not inhibited by adding Cd(2+) or cyclopiazonic acid but was inhibited by removing external Ca(2+). This indicates that this transient potentiation was dependent on external Ca(2+), but that this Ca(2+) influx was not via voltage-dependent Ca(2+) channels. KB-R7943, an inhibitor of NCX, at a concentration of 3 x 10(-6) M, reduced this transient increase of mIPSCs frequency without affecting mIPSCs amplitude and the response to exogenous GABA. These results demonstrate the existence of NCX in these GABAergic nerve terminals. In zero external Na(+), ouabain ...Continue Reading

References

Apr 1, 1991·The American Journal of Physiology·T NakagawaN Akaike
Jan 1, 1983·Annual Review of Physiology·R DiPolo, L Beaugé
Feb 15, 1995·The Journal of Physiology·K Kobayashi, M Tachibana
Oct 13, 1994·Nature·H Kamiya, R S Zucker
Apr 1, 1994·Journal of Neuroscience Methods·N AnkriH Korn
Jun 1, 1993·The Journal of Physiology·M Hoth, R Penner
Apr 15, 1996·Annals of the New York Academy of Sciences·S LitwinJ H Bridge
Mar 15, 1997·The Journal of Physiology·C R Rose, B R Ransom
Aug 1, 1998·Brain Research·M MunakataN Akaike
Aug 11, 1998·Neuron·M J Berridge
Oct 29, 1998·Biochemical and Biophysical Research Communications·M HironoT Yoshioka
Jul 3, 1999·Physiological Reviews·M P Blaustein, W J Lederer
Jul 3, 1999·Physiological Reviews·A MeirR Rahamimoff
Sep 2, 1999·The Journal of General Physiology·C C Lu, D W Hilgemann
Apr 13, 2000·The European Journal of Neuroscience·M JuhaszovaE F Stanley
Jun 1, 2000·The Journal of General Physiology·J P Danaceau, M T Lucero

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