NaCl but not urea activates p38 and jun kinase in mIMCD3 murine inner medullary cells

The American Journal of Physiology
Z Zhang, D M Cohen

Abstract

The mitogen-activated protein kinases (MAPKs), p38 and jun kinase (JNK), are activated by diverse stressors in cells of nonrenal medullary origin. Epithelial cells of the renal medulla are among the very few cells of higher eukaryotes routinely subjected to hyperosmotic stress, composed of principally NaCl and urea. Hyperosmotic NaCl activated p38 and JNK in a time- and dose-dependent fashion in cells of the murine terminal inner medullary collecting duct cell line (mIMCD3) as determined by immune complex kinase assay. Hyperosmotic urea exerted a minimal effect upon only p38 activation, which was evident only at 5 min. The NaCl effect was dose dependent to 800 mosmol/kgH2O; 800 mosmol/kgH2O urea, in contrast, exerted no effect. Consistent with these observations, NaCl (800 mosmol/kgH2O) but not urea (800 mosmol/kgH2O) increased tyrosine phosphorylation of p38 and JNK at 10 min. Therefore, even in the extremely osmotolerant renal medullary mIMCD3 cell line, derived from a tissue adapted for routine exposure to elevated osmolality, hypertonic NaCl activated two stress-responsive MAPKs. Urea, in contrast, exerted virtually no effect; therefore, cellular protection from urea stress operates through a mechanism distinct from the str...Continue Reading

Citations

Feb 8, 2000·American Journal of Physiology. Renal Physiology·Z ZhangD M Cohen
Sep 21, 2000·American Journal of Physiology. Renal Physiology·W TianD M Cohen
Jan 28, 2006·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Hiromi Tsushima, Mayumi Mori
Apr 9, 2001·American Journal of Physiology. Renal Physiology·J M CapassoT Berl
Mar 7, 2002·American Journal of Physiology. Renal Physiology·Hongyu ZhaoDavid M Cohen
Feb 20, 2009·American Journal of Physiology. Renal Physiology·Christoph KüperWolfgang Neuhofer
Aug 5, 2000·American Journal of Physiology. Renal Physiology·F X BeckE Müller
Feb 9, 2000·American Journal of Physiology. Cell Physiology·W TianD M Cohen
Oct 12, 2007·Physiological Reviews·Maurice B BurgNatalia I Dmitrieva
Jul 25, 2008·American Journal of Physiology. Cell Physiology·Pierre BissonnetteJean-Yves Lapointe
Oct 17, 2018·Cell Death Discovery·Arada VinaiphatVisith Thongboonkerd
Apr 9, 2001·American Journal of Physiology. Renal Physiology·W Tian, D M Cohen
Nov 1, 2002·American Journal of Physiology. Renal Physiology·Bento C SantosSteven R Gullans
Jun 27, 2014·American Journal of Physiology. Cell Physiology·Rong WangMaurice B Burg
Mar 23, 2002·Biochimica Et Biophysica Acta·Carole M Liedtke, Thomas S Cole

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