NAD+ analogs substituted in the purine base as substrates for poly(ADP-ribosyl) transferase

FEBS Letters
S L OeiM Ziegler

Abstract

Poly(ADP-ribosyl) transferase (pADPRT) catalyzes the transfer of the ADP-ribose moiety from NAD+ onto proteins as well as onto protein-bound ADP-ribose. As a result, protein-bound polymers of ADP-ribose are formed. pADPRT itself contains several acceptor sites for ADP-ribose polymers and may attach polymers to itself (automodification). In this study the influence of substitutions in the purine base of NAD+ on the polymerization reaction was investigated. The adenine moiety of NAD+ was replaced by either guanine, hypoxanthine or 1,N6-ethenoadenine. These analogs served as substrates for polymer synthesis as judged from the extent of automodification of the enzyme and the sizes of the polymers formed. Time course experiments revealed that 1,N6-etheno NAD+ (epsilon-NAD+) and nicotinamide hypoxanthine dinucleotide (NHD+) were rather poor substrates as compared to NAD+. Synthesis of GDP-ribose polymers from nicotinamide guanine dinucleotide (NGD+) was more efficient, but still significantly slower than poly(ADP-ribosyl)ation of the enzyme using NAD+. The size of the different polymers appeared to correlate with these observations. After 30 min of incubation in the presence of 1 mM substrate, polymers formed from epsilon-NAD+ or NHD...Continue Reading

References

Apr 25, 1990·Nucleic Acids Research·P L Panzeter, F R Althaus
Feb 1, 1986·Analytical Biochemistry·H J BurtscherM Hirsch-Kauffmann
Aug 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·J R BarrioN J Leonard
Sep 1, 1994·Molecular and Cellular Biochemistry·R Alvarez-GonzalezH Mendoza-Alvarez
Apr 1, 1994·Trends in Biochemical Sciences·G de Murcia, J Ménissier de Murcia
Oct 1, 1995·Trends in Biochemical Sciences·T LindahlA Klungland

❮ Previous
Next ❯

Citations

Nov 2, 2011·Chemical Communications : Chem Comm·Giulia PergolizziGerd K Wagner
Jul 12, 2001·Journal of Gastroenterology and Hepatology·N HigakiY Mochizuki
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Felicitas BergerMathias Ziegler
Sep 30, 2014·Proteomics·Christina A Vivelo, Anthony K L Leung
Mar 17, 2015·Nucleosides, Nucleotides & Nucleic Acids·Mikhail S Drenichev, Sergey N Mikhailov
Feb 20, 2007·Biochemical and Biophysical Research Communications·Masayasu ShiratoMitsuko Masutani
Nov 23, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Anais Depaix, Joanna Kowalska
Oct 7, 2020·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Junjie ZhuXiaochao Ma
Jun 12, 1999·Biochemical and Biophysical Research Communications·P Mayer-KuckukM Schweiger
Apr 15, 2004·Archives of Biochemistry and Biophysics·Claudia KeilShiao Li Oei

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.