PMID: 3585104Apr 1, 1987

NAD+-dependent 15-hydroxyprostaglandin dehydrogenase from the human term placenta

Nihon Sanka Fujinka Gakkai zasshi
T TanakaN Mori

Abstract

A NAD+-dependent 15-hydroxyprostaglandin dehydrogenase was purified from human term placenta using monoclonal antibody-coupled Sepharose affinity chromatography. The monoclonal antibodies were prepared against the molecule which was purified according to the method of Jarabak et al. About 4,800-fold purification was achieved with a 3% yield. The highest specific activity was 1,890 mU/mg. The molecular weight of the enzyme determined by gel filtration was 49,000. Polyacrylamide gel electrophoresis of the purified enzyme revealed the presence of two protein bands which were thought to be derived from the same enzyme molecule. Sodium dodecyl sulfate polyacrylamide gel electrophoresis showed two main bands of which the molecular weights were 28,000 to 30,000 and 52,000 to 56,000. Electrophoretic analyses suggest a labile oligomeric structure that may relate to the instability of the enzyme activity, which contradicts the monomer theory reported by Jarabak et al.

Related Concepts

Monoclonal Antibodies
Monoclonal antibodies, antineoplastic
Placenta Specimen
Hydroxyprostaglandin Dehydrogenases
Placenta
NADH
Gel Chromatography
Enzyme Activity
Blood Enzyme Activity (Lab Test)
MUROMONAB-CD3, Parenteral,5 MG Administered

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Synapse Loss as Therapeutic Target in MS

As we age, the number of synapses present in the human brain starts to decline, but in neurodegenerative diseases this occurs at an accelerated rate. In MS, it has been shown that there is a reduction in synaptic density, which presents a potential target for treatment. Here is the latest research on synapse loss as a therapeutic target in MS.

Artificial Intelligence in Cardiac Imaging

Artificial intelligence (ai) techniques are increasingly applied to cardiovascular (cv) medicine in cardiac imaging analysis. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Social Learning

Social learning involves learning new behaviors through observation, imitation and modeling. Follow this feed to stay up to date on the latest research.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Single Cell Chromatin Profiling

Techniques like ATAC-seq and CUT&Tag have the potential to allow single cell profiling of chromatin accessibility, histones, and TFs. This will provide novel insight into cellular heterogeneity and cell states. Discover the latest research on single cell chromatin profiling here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.