DOI: 10.1101/498162Dec 16, 2018Paper

NAD+ treatment can increase directly the antioxidant capacity of rotenone-treated differentiated PC12 cells

BioRxiv : the Preprint Server for Biology
Jie ZhangWeihai Ying

Abstract

NAD+ administration can produce profound beneficial effects in the animal models of aging and a number of diseases. Since oxidative stress plays key pathological roles in aging and multiple major disorders, it is crucial to elucidate the mechanisms underlying the protective effects of NAD+ administration on oxidative stress-induced cell death. Previous studies have suggested that NAD+ treatment can decrease oxidative cell death indirectly by such mechanisms as preventing mitochondrial permeability transition, while it is unclear if NAD+ administration may decrease oxidative cell death by increasing directly the antioxidant capacity of the cells. Our current study used rotenone-treated differentiated PC12 cells as a cellular model to test our hypothesis that NAD+ treatment may increase directly the antioxidant capacity of the cells exposed to oxidative stress. Our study has indicated that NAD+ treatment can significantly attenuate the rotenone-induced increase in oxidative stress in the cells. Moreover, NAD+ treatment can significantly enhance the GSH/GSSG ratio, a major index of antioxidant capacity, of rotenone-treated cells. Collectively, our study has provided the first evidence indicating that NAD+ treatment can increase di...Continue Reading

Related Concepts

Aging
Antioxidants
Glutathione
Nicotinamide adenine dinucleotide (NAD)
Rotenone
PC12 Cells
Oxidative Stress
Disease Model
Administration Procedure
Study

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