1. Both NADH and NADPH supported the oxidation of adrenaline to adrenochrome in bovine heart submitochondrial particles. The reaction was completely inhibited in the presence of superoxide dismutase, suggesting that superoxide anions (O(2) (-)) are responsible for the oxidation. The optimal pH of the reaction with NADPH was at pH7.5, whereas that with NADH was at pH9.0. The reaction was inhibited by treatment of the preparation with p-hydroxymercuribenzoate and stimulated by treatment with rotenone. Antimycin A and cyanide stimulated the reaction to the same extent as rotenone. The NADPH-dependent reaction was inhibited by inorganic salts at high concentrations, whereas the NADH-dependent reaction was stimulated. 2. Production of O(2) (-) by NADH-ubiquinone reductase preparation (Complex I) with NADH or NADPH as an electron donor was assayed by measuring the formation of adrenochrome or the reduction of acetylated cytochrome c which does not react with the respiratory-chain components. p-Hydroxymercuribenzoate inhibited the reaction and rotenone stimulated the reaction. The effects of pH and inorganic salts at high concentrations on the NADH- and NADPH-dependent reactions of Complex I were essentially similar to those on the re...Continue Reading
Spin-trapping and direct EPR investigations on the hepatotoxic and hepatocarcinogenic actions of luteoskyrin, an anthraquinoid mycotoxin produced by Penicillium islandicum Sopp. Generations of superoxide anion and luteoskyrin semiquinone radical in the redox systems consisted of luteoskyrin and liver NADPH- or NADH-dependent reductases
Prohibitin-1 maintains the angiogenic capacity of endothelial cells by regulating mitochondrial function and senescence
Reactive oxygen species (ROS)-induced ROS release: a new phenomenon accompanying induction of the mitochondrial permeability transition in cardiac myocytes
Molecular identification of the enzyme responsible for the mitochondrial NADH-supported ammonium-dependent hydrogen peroxide production
Mechanism of cytotoxicity of paraquat. I. NADH oxidation and paraquat radical formation via complex I
Evidence of ROS generation by mitochondria in cells with impaired electron transport chain and mitochondrial DNA damage
Effect of monovalent cations on the kinetics of hypoxic conformational change of mitochondrial complex I
Aging in vertebrates, and the effect of caloric restriction: a mitochondrial free radical production-DNA damage mechanism?
Short-term caloric restriction and sites of oxygen radical generation in kidney and skeletal muscle mitochondria
Wound-induced apoplastic peroxidase activities: their roles in the production and detoxification of reactive oxygen species
Extracellular superoxide production, viability and redox poise in response to desiccation in recalcitrant Castanea sativa seeds
A proposed interplay between peroxidase, amine oxidase and lipoxygenase in the wounding-induced oxidative burst in Pisum sativum seedlings
Mitochondrial hydrogen peroxide production as determined by the pyridine nucleotide pool and its redox state
New insights into the superoxide generation sites in bovine heart NADH-ubiquinone oxidoreductase (Complex I): the significance of protein-associated ubiquinone and the dynamic shifting of generation sites between semiflavin and semiquinone radicals
Human complex I deficiency: clinical spectrum and involvement of oxygen free radicals in the pathogenicity of the defect
Interaction of genistein with the mitochondrial electron transport chain results in opening of the membrane transition pore
Modification of glycolysis affects cell sensitivity to apoptosis induced by oxidative stress and mediated by mitochondria
An 808-nm Diode Laser with a Flat-Top Handpiece Positively Photobiomodulates Mitochondria Activities
Vulnerability to oxidative stress in vitro in pathophysiology of mitochondrial short-chain acyl-CoA dehydrogenase deficiency: response to antioxidants
Divergent targets of glycolysis and oxidative phosphorylation result in additive effects of metformin and starvation in colon and breast cancer
Mitochondrial signal lacking manganese superoxide dismutase failed to prevent cell death by reoxygenation following hypoxia in a human pancreatic cancer cell line, KP4
Regulation of manganese superoxide dismutase in Saccharomyces cerevisiae. The role of respiratory chain activity
Comparison of oxidant-generation and BP-diol activation by bone marrow cells from C57Bl/6 and DBA/2 mice: implications for risk of bone marrow toxicity induced by polycyclic hydrocarbons
An oxidative burst of superoxide in embryonic axes of recalcitrant sweet chestnut seeds as induced by excision and desiccation
Possible involvement of the alpha1 isoform of 5'AMP-activated protein kinase in oxidative stress-stimulated glucose transport in skeletal muscle
Opening of the mitochondrial permeability transition pore induces reactive oxygen species production at the level of the respiratory chain complex I
Intracellular localization of 8-oxo-dGTPase in human cells, with special reference to the role of the enzyme in mitochondria
Hypochlorite modified albumins promote cell death in the tubule interstitium in rats via mitochondrial damage in obstructive nephropathy and the protective effects of antioxidant peptides
Structure and organization of mitochondrial respiratory complexes: a new understanding of an old subject
Updating the mitochondrial free radical theory of aging: an integrated view, key aspects, and confounding concepts
MIBG causes oxidative stress and up-regulation of anti-oxidant enzymes in the human neuroblastoma cell line SK-N-BE(2c)
Mitochondrial electron transport complex I is a potential source of oxygen free radicals in the failing myocardium
Acylation of Superoxide Dismutase 1 (SOD1) at K122 Governs SOD1-Mediated Inhibition of Mitochondrial Respiration
Superoxide radical formation by pure complex I (NADH:ubiquinone oxidoreductase) from Yarrowia lipolytica
The nature and mechanism of superoxide production by the electron transport chain: Its relevance to aging
NADH-coenzyme Q reductase (complex I) deficiency: heterogeneity in phenotype and biochemical findings
Protein and lipid oxidative damage and complex I content are lower in the brain of budgerigar and canaries than in mice. Relation to aging rate
Gastric acid induces mitochondrial superoxide production and lipid peroxidation in gastric epithelial cells
Roles of mitochondria-generated reactive oxygen species on X-ray-induced apoptosis in a human hepatocellular carcinoma cell line, HLE
The parkinsonism-inducing drug 1-methyl-4-phenylpyridinium triggers intracellular dopamine oxidation. A novel mechanism of toxicity
Hepatitis C virus core protein inhibits mitochondrial electron transport and increases reactive oxygen species (ROS) production
DeltaPsi(m)-Dependent and -independent production of reactive oxygen species by rat brain mitochondria
Oxidative stress in myelin sheath: The other face of the extramitochondrial oxidative phosphorylation ability
Effects of a high-fat diet on superoxide anion generation and membrane fluidity in liver mitochondria in rats
Vitamin E Supplementation and Mitochondria in Experimental and Functional Hyperthyroidism: A Mini-Review
Cell-Permeable Calpain Inhibitor SJA6017 Provides Functional Protection to Spinal Motoneurons Exposed to MPP
The content of intracellular mitochondrial DNA is decreased by 1-methyl-4-phenylpyridinium ion (MPP+)
Role of the prosthetic groups of NADPH-cytochrome P450 reductase in 3-hydroxyanthranilamide-catalyzed, NADPH-dependent superoxide anion production
Modulation of the conformational state of mitochondrial complex I as a target for therapeutic intervention
Mitochondrial Biogenesis and Mitochondrial Reactive Oxygen Species (ROS): A Complex Relationship Regulated by the cAMP/PKA Signaling Pathway
Mitochondrial function in Parkinson's disease. The Royal Kings and Queens Parkinson's Disease Research Group
High rates of superoxide production in skeletal-muscle mitochondria respiring on both complex I- and complex II-linked substrates
Psi-screen, an in vitro toxicity test system: applications in the bioassay of perfumes and fragrance chemicals
The Role of Mitochondria in Reactive Oxygen Species Generation and Its Implications for Neurodegenerative Diseases
Silicon in Horticultural Crops: Cross-talk, Signaling, and Tolerance Mechanism under Salinity Stress
Colonic Oxidative and Mitochondrial Function in Parkinson's Disease and Idiopathic REM Sleep Behavior Disorder
Neuroprotective action of Eicosapentaenoic (EPA) and Docosahexaenoic (DHA) acids on Paraquat intoxication in Drosophila melanogaster
Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.
Synthetic Genetic Array Analysis
Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.
Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.
Neural Activity: Imaging
Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.
Chronic Fatigue Syndrome
Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.
Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.
Cell Atlas of the Human Eye
Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.
Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.
STING Receptor Agonists
Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.