NADH Dehydrogenases in Pseudomonas aeruginosa Growth and Virulence

Frontiers in Microbiology
Angela TorresAnna Zemke

Abstract

Pseudomonas aeruginosa is an opportunistic human pathogen with a complex respiratory chain. The bacterium is predicted to express three NADH:ubiquinone oxidoreductases (NDH-1, NDH-2 and Nqr). We created deletions strains of the predicted NADH:ubiquinone oxidoreductases alone, and in combination to determine the respective roles of the NADH dehydrogenases in growth and virulence. NDH-1 and NDH-2 were largely redundant under aerobic conditions. Aerobic NADH dehydrogenase enzymatic activity assay was lost with deletion of both NDH-1 and NDH-2. Under anaerobic conditions, NDH-1 was required for robust growth, and overexpression of NDH-2 rescued the NDH-1 anaerobic growth defect in rich media. There was not compensatory upregulation of NDH-2 under anaerobic conditions in NDH-1 deletion strains. To test which genes were required for in vivo virulence, we used both an insect and plant disease model. In the Galleria mellonella model, neither deletion of NDH-1 nor NDH-2 led to a change in median lethal dose, although death occurred more slowly in the NDH-1 deletion infections. In a lettuce model of virulence, loss of NDH-1 caused a decrease in recovered viable bacteria and a decrease in visual tissue damage. The compound deletion of NDH...Continue Reading

References

Dec 26, 2001·Nucleic Acids Research·Ron EdgarAlex E Lash
Jul 18, 2002·Molecular Plant-microbe Interactions : MPMI·Margarita M Camacho CarvajalGuido V Bloemberg
Jun 23, 2006·Infection and Immunity·Melanie J FiliatraultBarbara H Iglewski
Jul 6, 2006·Applied and Environmental Microbiology·Robert M Q ShanksGeorge A O'Toole
Apr 5, 2007·Nature Protocols·Kyoung-Hee Choi, Herbert P Schweizer
May 22, 2007·Results and Problems in Cell Differentiation·Stefan KerscherUlrich Brandt
Oct 1, 2008·Antimicrobial Agents and Chemotherapy·Kristen N SchurekRobert E W Hancock
Jan 31, 2009·Nature Protocols·Melissa Starkey, Laurence G Rahme
Nov 26, 2009·Environmental Microbiology·Takuro KawakamiHiroyuki Arai
Jun 18, 2010·Environmental Microbiology·Katharina TrunkDieter Jahn
Oct 27, 2011·PLoS Pathogens·Sasan AminiSaeed Tavazoie
Oct 10, 2012·Biochimica Et Biophysica Acta·Jianwei Chen, Marc Strous
Mar 29, 2014·The New England Journal of Medicine·Shelley S MagillUNKNOWN Emerging Infections Program Healthcare-Associated Infections and Antimicrobial Use Prevalence Survey Team
May 20, 2014·Methods in Molecular Biology·Daniel E Deatherage, Jeffrey E Barrick
Dec 6, 2014·Advances in Microbial Physiology·Gregory M CookMichael Berney
Sep 6, 2015·Journal of Microbiological Methods·Angela Glassing, Thomas A Lewis
Oct 23, 2015·Nature Protocols·Laura R HmeloJoe J Harrison
Feb 3, 2016·Journal of Bacteriology·Melanie A SperoTimothy J Donohue
Mar 14, 2018·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Matthew R CrullChristopher H Goss

❮ Previous
Next ❯

Citations

May 16, 2020·The Journal of Biological Chemistry·Chau-Duy-Tam VoLudovic Pelosi
Nov 1, 2020·Nature Communications·Paul O SheridanCécile Gubry-Rangin
Feb 1, 2021·The Journal of Biological Chemistry·Chau-Duy-Tam VoLudovic Pelosi

❮ Previous
Next ❯

Software Mentioned

Prism
GraphPad
GEO2R
Breseq

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.