NADH-dependent glutamate synthase participated in ammonium assimilation in Arabidopsis root

Plant Signaling & Behavior
Soichi KojimaT Yamaya

Abstract

Higher plants have 2 GOGAT species, Fd-GOGAT and NADH-GOGAT. While Fd-GOGAT mainly assimilates ammonium in leaves, which is derived from photorespiration, the function of NADH-GOGAT, which is highly expressed in roots, (1) needs to be elucidated. The aim of this study was to clarify the role of NADH-GOGAT in Arabidopsis roots. The supply of ammonium to the roots caused an accumulation of NADH-GOGAT, while Fd-GOGAT 1 and Fd-GOGAT 2 showed no response. A promoter-GUS fusion analysis and immunohistochemistry showed that NADH-GOGAT was located in non-green tissues like vascular bundles, shoot apical meristem, pollen, stigma, and roots. The localization of NADH-GOGAT and Fd-GOGAT was not overlapped. NADH-GOGAT T-DNA insertion lines showed a reduction of glutamate and biomass under normal CO2 conditions. These data emphasizes the importance of NADH-GOGAT in the ammonium assimilation of Arabidopsis roots.

References

Oct 18, 1974·Nature·P J Lea, B J Miflin
Jul 1, 1995·The Plant Cell·N M Crawford
Feb 15, 2002·The Plant Journal : for Cell and Molecular Biology·Muriel LancienGloria M Coruzzi
Mar 26, 2002·Journal of Experimental Botany·J K SchjoerringM Mattsson
Jun 1, 1996·Annual Review of Plant Physiology and Plant Molecular Biology·H.-M. LamG. M. Coruzzi
Jun 21, 2007·Journal of Experimental Botany·Brian G Forde, Peter J Lea
Mar 20, 2013·Plant & Cell Physiology·Kazuhiro FunayamaTomoyuki Yamaya

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