PMID: 429212Feb 26, 1979Paper

NADP-dependent dehydrogenases in rat liver parenchyma. II. Comparison of qualitative and quantitative G6PDH distribution patterns with particular reference to sex differences

Histochemistry
H F Teutsch, H Rieder

Abstract

Qualitative histochemical G6PDH distribution patterns obtained in the liver acinus of adult male and female rats with an improved method (Rieder et al., 1978) served as a basis for the isolation by microdissection of tissue samples of defined zonal affiliation. G6PDH activity was assayed quantitatively in tissue samples of zones 1 and 3 by a microfluorometric method, using the oil well technique and enzymatic cycling (Burch et al., 1963; Lowry and Passonneau, 1972). With the use of a correlation system further evidence could be presented for the validity of the recently described qualitative distribution patterns. From a total of 50 analyzed tissue samples the following G6PDH activities were calculated: 4.25 +/- 1.56 U/g dry weight in zone 1 and 2.08 +/- 0.46 U/g dry weight in zone 3 of male and 7.21 +/- 1.03 U/g dry weight in zone 1 and 11.10 +/- 2.56 U/g dry weight in zone 3 of female rats. These data were corrected for interference from the G6PDH activity of the Kupffer cells within zone 1 samples (approximately 80 U/g dry weight), so that the actual relative values for the parenchymal activity could be estimated for the first time: 2 U/g dry weight in zones 1 and 3 of male animals, 5 U/g dry weight in zone 1 and 11 U/g dry ...Continue Reading

References

Feb 15, 1979·Cell and Tissue Research·W HosemannD Sasse
Oct 15, 1977·Experimental Cell Research·D L KnookE C Sleyster
Sep 1, 1967·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S H Hori, S I Matsui
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Jul 1, 1959·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R E SHANKR SCHWARTZ
Jul 1, 1959·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A B NOVIKOFF
Feb 1, 1964·The American Journal of Physiology·H M TEPPERMAN, J TEPPERMAN

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Citations

Jan 1, 1991·Archives of Andrology·P SiriguE S Hafez
Jan 1, 1980·Histochemistry·R NuberD Sasse
Dec 28, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Wilma M FrederiksCornelis J F Van Noorden
May 1, 1990·Journal of Gastroenterology and Hepatology·Y Uchiyama
Jan 1, 1985·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·R G Thurman, F C Kauffman
Sep 1, 1989·The Histochemical Journal·N R Katz
Jul 1, 1984·The Histochemical Journal·C J van NoordenJ James
Mar 1, 1989·European Journal of Biochemistry·N KatzS Giffhorn-Katz
May 1, 1982·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·K Jungermann, N Katz
Dec 1, 1985·Experimental Cell Research·C J Van NoordenJ James

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