NADP-Malate Dehydrogenase of Sweet Sorghum Improves Salt Tolerance of Arabidopsis thaliana

Journal of Agricultural and Food Chemistry
Yuanyuan GuoNa Sui

Abstract

Sweet sorghum is a C4 crop that shows high salt tolerance and high yield. NADP-malate dehydrogenase ( NADP-ME) is a crucial enzyme of the C4 pathway. The regulatory mechanism of NADP-ME remains unclear. In this study, we isolated SbNADP-ME from sweet sorghum. The open reading frame of SbNADP-ME is 1911 bp and 637 amino acid residues. Quantitative real-time PCR analysis showed that SbNADP-ME transcription in sweet sorghum was enhanced by salt stress. The SbNADP-ME transcript level was highest under exposure to 150 mM NaCl. Arabidopsis overexpressing SbNADP-ME showed increased germination rate and root length under NaCl treatments. At the seedling stage, physiological photosynthesis parameters, chlorophyll content, PSII photochemical efficiency, and PSI oxidoreductive activity in the wild type decreased more severely than in the overexpression lines but less than in T-DNA insertion mutants under salt stress. Overexpression of SbNADP-ME in Arabidopsis may also increase osmotic adjustment and scavenging activity on DPPH and decrease membrane peroxidation. These results suggest that SbNADP-ME overexpression in Arabidopsis increases salt tolerance and alleviates PSII and PSI photoinhibition under salt stress by improving photosynthet...Continue Reading

References

Mar 12, 2002·Journal of Experimental Botany·Rainer E HäuslerChristoph Peterhänsel
Mar 12, 2002·Journal of Experimental Botany·Marianne M LaporteMitchell C Tarczynski
Jul 10, 2002·Annals of Botany·M M ChavesC Pinheiro
Oct 30, 2003·Journal of Agricultural and Food Chemistry·Joseph M AwikaLuis Cisneros-Zevallos
Apr 14, 2004·Journal of Plant Physiology·Hua-Xin ChenHui-Yuan Gao
May 7, 2005·Archaea : an International Microbiological Journal·Wakao FukudaTadayuki Imanaka
Jun 21, 2006·Biochimica Et Biophysica Acta·Yoshitaka NishiyamaNorio Murata
Jan 9, 2007·Biochimica Et Biophysica Acta·Norio MurataSuleyman I Allakhverdiev
Aug 31, 2007·Plant, Cell & Environment·Jaume FlexasMiquel Ribas-Carbo
May 1, 2008·Annual Review of Plant Biology·Rana Munns, Mark Tester
Jan 24, 2009·Trends in Plant Science·Wojciech Majeran, Klaas J van Wijk
Feb 4, 2009·Nature·Andrew H PatersonDaniel S Rokhsar
Jul 2, 2010·Plant Signaling & Behavior·Sarvajeet Singh Gill, Narendra Tuteja
Sep 1, 1990·Photosynthesis Research·O van Kooten, J F Snel
Oct 18, 2015·Plant Science : an International Journal of Experimental Plant Biology·Mariana B BadiaMariel C Gerrard Wheeler
Jan 28, 2017·Journal of Agricultural and Food Chemistry·Hyun Jeong LeeHan Min Woo
Dec 2, 2017·Journal of Agricultural and Food Chemistry·Amalyn Nain-PerezGiuseppe Forlani

❮ Previous
Next ❯

Citations

Jul 20, 2019·Plant Signaling & Behavior·Xi SunNa Sui
Nov 21, 2019·International Journal of Molecular Sciences·Jianbo LiHuixia Jia
Nov 8, 2018·International Journal of Molecular Sciences·Qian-Feng LiQiao-Quan Liu
Aug 8, 2021·International Journal of Molecular Sciences·Guoliang HanBaoshan Wang
Jan 5, 2022·International Journal of Radiation Biology·Madhavi V PurankarSuprasanna Penna

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.