Sep 1, 1987

NADP-specific isocitrate dehydrogenase of Mycobacterium phlei ATCC 354: purification and characterization

Journal of General Microbiology
K R Dhariwal, T A Venkitasubramanian


NADP-dependent isocitrate dehydrogenase (EC from Mycobacterium phlei ATCC 354 was purified to homogeneity by ammonium sulphate fractionation, followed by DEAE cellulose and Sephadex G-200 chromatography. The pH optimum of the enzyme was 8.5. The Km values for isocitrate and NADP were 74 and 53 microM, respectively. Mn2+ was essential for enzyme activity. The enzyme lost all activity on incubation at 70 degrees C for 15 min; isocitrate and NADP protected against this thermal inactivation. p-Chloromercuribenzoate inhibited the enzyme; pre-incubation of enzyme with isocitrate + Mn2+ prevented this inhibition. The purified enzyme showed concerted inhibition by glyoxylate + oxaloacetate and was inhibited by oxalomalate.

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Mentioned in this Paper

Sephadex G 200
Idp1 protein, S pombe
Isocitrate Dehydrogenase-I
Enzyme Activity

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