PMID: 36923May 23, 1979

NADP-specific isocitrate dehydrogenase of Escherichia coli. IV. Purification by chromatography on Affi-Gel Blue

Biochimica Et Biophysica Acta
B Vasquez, H C Reeves

Abstract

Affinity chromatography on Affi-Gel Blue has been used to purify the NADP-specific isocitrate dehydrogenase (EC 1.1.1.42) from Escherichia coli. The protocol permits rapid purification of the enzyme in milligram quantities with a yield of 50% and is carried out almost entirely at room temperature. The preparation was judged to be homogeneous by non-denaturing electrophoresis at pH 7.5 and denaturing electrophoresis in the presence of sodium dodecyl sulfate. The subunit molecular weight of 53 000, determined by sodium dodecyl sulfate gel electrophoresis, is in reasonable agreement with the value of 46 900 estimated from the amino acid composition data.

Citations

Jan 19, 1999·Annual Review of Microbiology·A J Cozzone
Nov 26, 2009·Critical Reviews in Biochemistry and Molecular Biology·Daniel N Wilson
Aug 14, 1981·Biochemical and Biophysical Research Communications·D UrsicJ P Helgeson
Oct 1, 1982·Archives of Biochemistry and Biophysics·J Y Wang, D E Koshland
Mar 22, 1990·Journal of Theoretical Biology·K R AlbeB E Wright
Jun 1, 1984·European Journal of Biochemistry·A C BorthwickH G Nimmo
Sep 1, 1980·European Journal of Biochemistry·D PomponF Lederer
Jan 12, 2019·Frontiers in Bioengineering and Biotechnology·Reinhard OegglDörte Rother
Nov 2, 2001·Journal of Biomolecular Screening·U SchwanebergF H Arnold

Related Concepts

Chromatography, Affinity
Alkalescens-Dispar Group
Isocitrate Dehydrogenase-I
NADP
Substrate Specificity

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