journal cover

NADP+-specific isocitrate dehydrogenase of Excherichia coli. III. Two-step purification employing affinity chromatography

Biochimica Et Biophysica Acta

Sep 14, 1976

M Hy, H C Reeves

PMID: 8141

Abstract

The NADP+-specific isocitrate dehydrogenase (threo-DS-isocitrate:NADP+ oxidoreductase (decarboxylating), EC 1.1.1.42) of Excherichia coli has been purified to electrophoretic homogeneity by a two-step purification procedure employing affinity chromatography. The overall yield of enzyme ...read more

Mentioned in this Paper

Phosphate buffers
Alkalescens-Dispar Group
Polyacrylamide Gels
Isocitrate
Tromethamine
Citrate Measurement
Isocitrate Dehydrogenase-I
Purification Aspects
IDH2
NADP

NADP+-specific isocitrate dehydrogenase of Excherichia coli. III. Two-step purification employing affinity chromatography

Biochimica Et Biophysica Acta

Sep 14, 1976

M Hy, H C Reeves

PMID: 8141

DOI:

Abstract

The NADP+-specific isocitrate dehydrogenase (threo-DS-isocitrate:NADP+ oxidoreductase (decarboxylating), EC 1.1.1.42) of Excherichia coli has been purified to electrophoretic homogeneity by a two-step purification procedure employing affinity chromatography. The overall yield of enzyme ...read more

Mentioned in this Paper

Phosphate buffers
Alkalescens-Dispar Group
Polyacrylamide Gels
Isocitrate
Tromethamine
Citrate Measurement
Isocitrate Dehydrogenase-I
Purification Aspects
IDH2
NADP
/papers/nadp-specific-isocitrate-dehydrogenase-of/8141