NADPH-Cytochrome P450 Reductase Mediates the Resistance of Aphis (Toxoptera ) citricidus (Kirkaldy) to Abamectin

Frontiers in Physiology
Tian-Xing JingJin-Jun Wang

Abstract

NADPH-cytochrome P450 reductase (CPR) plays an essential role in the cytochrome P450 enzyme system, which aids in the metabolism of endogenous and exogenous compounds including the detoxification of insecticides. In this study, the CPR transcript in Aphis (Toxoptera) citricidus (Kirkaldy) was cloned, and the deduced amino acid sequence contained an N-terminal membrane anchor, three conserved binding domains (flavin mononucleotide, flavin adeninedinucleotide, and nicotinamide adenine dinucleotide phosphate), a flavin adeninedinucleotide-binding motif, and catalytic residues. Based on phylogenetic analysis, AcCPR was grouped in the hemipteran branch. AcCPR was ubiquitously expressed at all developmental stages and was most abundant in the adults and least abundant in third instar nymphs. Compared with other tested tissues of adults, the expression level of AcCPR was significantly high in the gut. Feeding double-stranded RNA of AcCPR reduced the AcCPR mRNA level and the activity of AcCPR in aphids, and the treated insects exhibited higher susceptibility to abamectin than the control group. Furthermore, the heterologous overexpression of AcCPR in Sf9 cells resulted in a greater viability than control cells when treated with abamect...Continue Reading

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Citations

Oct 28, 2019·International Journal of Molecular Sciences·Lifang SunJianguo Xu
Oct 22, 2020·The Journal of Animal Ecology·Cristina BotíasDave Goulson
Mar 28, 2021·Pesticide Biochemistry and Physiology·Li ShiXiaolan Liao

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Methods Mentioned

BETA
PCR

Software Mentioned

TMHMM
DNAMAN
MEGA
SPSS
ExPASy Proteomics Server
PRIMER

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