PMID: 8943120Nov 1, 1996Paper

NADPH-diaphorase histochemistry: a reliable test for the intraoperative diagnosis of Hirschsprung's disease

Journal of Pediatric Surgery
Hiroyuki KobayashiP Puri

Abstract

Seromuscular biopsies were obtained from eight cases, at various levels, during a Swanson pull-through operation. The specimens were examined by using NADPH-diaphorase histochemistry. In the ganglionic segment of bowel, there were a large number of NADPH-diaphorase-positive nerve fibers in the circular and longitudinal muscles, and the myenteric ganglia stained strongly. In the transitional segment of bowel, there were a few NADPH-diaphorase-positive nerve fibers in the circular and longitudinal muscles, and the myenteric ganglion cells were smaller. In the aganglionic segment of bowel, there was a lack of expression of NADPH-diaphorase in the circular and longitudinal muscles. Hypertrophied nerve trunks in the myenteric plexus were stained. NADPH-diaphorase histochemistry is a simple, reliable, and rapid method for the intraoperative evaluation of the aganglionic segment of bowel. This technique allows intraoperative demonstration of innervation patterns of smooth muscle in normal and HD bowel, and provides accurate identification of ganglion cells in the ganglionic segment.

References

Apr 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·B T HopeS R Vincent
Dec 30, 1993·The New England Journal of Medicine·S Moncada, A Higgs

❮ Previous
Next ❯

Citations

Jan 16, 1999·Pediatric Clinics of North America·R H PearlP L Glick
Mar 26, 2002·Journal of Pediatric Surgery·Udo RollePrem Puri

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.