NADPH-flavodoxin reductase and flavodoxin from Escherichia coli: characteristics as a soluble microsomal P450 reductase

Biochemistry
C M Jenkins, M R Waterman

Abstract

In addition to their endogenous roles as an activation system for various Escherichia coli metabolic pathways, the soluble flavoproteins flavodoxin (Fld) and NADPH-flavodoxin (ferredoxin) reductase (Fpr) can serve as an electron-transfer system for microsomal cytochrome P450s. Furthermore, since Fld and Fpr are structurally similar to the functional domains (FMN binding and NADPH/FAD binding domains, respectively) of NADPH-cytochrome P450 reductases (P450 reductases), these bacterial proteins represent a potentially useful model system for eukaryotic P450 reductases. Here we delineate similarities and differences between the E. coli Fpr-Fld system and rat P450 reductase as electron donors to bovine 17alpha-hydroxylase/17,20-lyase P450 (P450c17). Importantly, recombinant Fpr, in combination with recombinant Fld, supports both the hydroxylase and lyase activities of P450c17 to the same proportional extent (hydroxylase-to-lyase ratio) as does P450 reductase. Maximum P450c17 turnover [5-6 mol of 17alpha-OH-progesterone (mol of P450c17)-1 min-1] was achieved using a large molar excess (50-100-fold over P450c17) of a 1:1 ratio of Fpr-Fld, although this rate was an order of magnitude less than the maximal P450 reductase-supported acti...Continue Reading

References

Sep 3, 1990·Biochimica Et Biophysica Acta·M F FillatC Gomez-Moreno
Mar 1, 1971·Archives of Biochemistry and Biophysics·A Hildebrandt, R W Estabrook

❮ Previous
Next ❯

Citations

Feb 27, 2010·Applied Microbiology and Biotechnology·Marco GirhardVlada B Urlacher
Nov 17, 2010·Applied Microbiology and Biotechnology·Sangkyu ParkKyoungwhan Back
Mar 31, 2012·Applied Microbiology and Biotechnology·Ming JiangBlaine A Pfeifer
Apr 6, 2004·Biochimica Et Biophysica Acta·Marat B MuratalievF Ann Walker
Sep 18, 2002·Archives of Biochemistry and Biophysics·Jason T Wan, Joseph T Jarrett
Jan 11, 2001·Biochimica Et Biophysica Acta·C S MilesS K Chapman
Dec 18, 2001·Biosensors & Bioelectronics·Gianfranco GilardiSilva Giannini
Nov 7, 2002·Trends in Microbiology·René De Mot, Annabel H A Parret
Aug 9, 2001·Proceedings of the National Academy of Sciences of the United States of America·D A HallR G Matthews
Nov 25, 2004·European Journal of Biochemistry·Daniela L Catalano DupuyEduardo A Ceccarelli
Jul 29, 2008·Journal of Bacteriology·Sean A AggerClaudia Schmidt-Dannert
Oct 27, 2010·PloS One·María G MediavillaClaudio Tiribelli
Feb 13, 2001·Biochemical and Biophysical Research Communications·M A KaderbhaiS L Kelly
May 23, 2014·Applied Microbiology and Biotechnology·Rita Bernhardt, Vlada B Urlacher
Jan 6, 2004·Proceedings of the National Academy of Sciences of the United States of America·Li LeiDavid C Lamb
Mar 5, 1999·Drug Metabolism Reviews·C M Jenkins, M R Waterman
Jun 9, 2004·Chembiochem : a European Journal of Chemical Biology·Kevin T WattsClaudia Schmidt-Dannert
Apr 27, 2010·Chembiochem : a European Journal of Chemical Biology·David B HawkesJames J De Voss
Aug 21, 2007·Biochemical and Biophysical Research Communications·Marco GirhardVlada B Urlacher
Nov 22, 2005·Biochemical and Biophysical Research Communications·Kyoko MomoiVlada B Urlacher
Sep 17, 2015·Microbial Cell Factories·Simone Brixius-AnderkoRita Bernhardt
May 28, 2011·Journal of Biological Engineering·Buz BarstowEdwin H Wintermute
Aug 18, 2004·Journal of Lipid Research·Aouatef BellamineMichael R Waterman
Dec 22, 2017·Chembiochem : a European Journal of Chemical Biology·Ansgar RühlmannVlada B Urlacher
Mar 8, 2018·Chembiochem : a European Journal of Chemical Biology·Bikash DangiTae-Jin Oh

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biosynthetic Transformations

Biosyntheic transformtions are multi-step, enzyme-catalyzed processes where substrates are converted into more complex products in living organisms. Simple compounds are modified, converted into other compounds, or joined together to form macromolecules. Discover the latest research on biosynthetic transformations here.