NADPH oxidase 2 plays a role in experimental corneal neovascularization

Clinical Science
Elsa C ChanGuei-Sheung Liu

Abstract

Corneal neovascularization, the growth of new blood vessels in the cornea, is a leading cause of vision impairment after corneal injury. Neovascularization typically occurs in response to corneal injury such as that caused by infection, physical trauma, chemical burns or in the setting of corneal transplant rejection. The NADPH oxidase enzyme complex is involved in cell signalling for wound-healing angiogenesis, but its role in corneal neovascularization has not been studied. We have now analysed the role of the Nox2 isoform of NADPH oxidase in corneal neovascularization in mice following chemical injury. C57BL/6 mice aged 8-14 weeks were cauterized with an applicator coated with 75% silver nitrate and 25% potassium nitrate for 8 s. Neovascularization extending radially from limbal vessels was observed in corneal whole-mounts from cauterized wild type mice and CD31+ vessels were identified in cauterized corneal sections at day 7. In contrast, in Nox2 knockout (Nox2 KO) mice vascular endothelial growth factor-A (Vegf-A), Flt1 mRNA expression, and the extent of corneal neovascularization were all markedly reduced compared with their wild type controls. The accumulation of Iba-1+ microglia and macrophages in the cornea was signifi...Continue Reading

References

Jun 30, 2004·Investigative Ophthalmology & Visual Science·Steven E WilsonJuan P Rodriguez-Perez
Jun 28, 2005·Investigative Ophthalmology & Visual Science·Gabriel SosneRafael Fridman
Jan 21, 2006·The British Journal of Ophthalmology·T OshimaT Ishibashi
Jun 20, 2006·Cardiovascular Research·Masuko Ushio-Fukai
Dec 21, 2006·The British Journal of Ophthalmology·Roberta P A ManzanoPatricia Chévez-Barrios
Jun 27, 2007·Cornea·Mesut ErdurmusRemzi Karadag
Apr 2, 2008·Investigative Ophthalmology & Visual Science·Mohamed Al-ShabraweyRuth B Caldwell
Apr 29, 2008·Ophthalmology·Sang Woo KimTae-im Kim
Sep 12, 2008·Antioxidants & Redox Signaling·Randall S FreyAsrar B Malik
Nov 18, 2008·Journal of Cellular and Molecular Medicine·F JiangG J Dusting
Sep 18, 2010·Investigative Ophthalmology & Visual Science·Miyuki KubotaKazuo Tsubota
Mar 11, 2011·Cornea·Deepak Gupta, Chris Illingworth
Jun 15, 2011·Investigative Ophthalmology & Visual Science·Deniz HosClaus Cursiefen
May 15, 2012·Circulation Research·Bernard LassègueKathy K Griendling
Aug 18, 2012·Survey of Ophthalmology·Jin-Hong ChangDimitri T Azar
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Sep 13, 2012·American Journal of Ophthalmology·Sheng-Fu ChengReza Dana
Feb 6, 2013·PloS One·Sanjay ChaubeyClaire M Wells
Sep 24, 2013·Antioxidants & Redox Signaling·Jennifer L Wilkinson-BerkaHarald H H W Schmidt
Oct 10, 2013·Investigative Ophthalmology & Visual Science·Elsa C ChanGregory J Dusting
Nov 5, 2013·Antioxidants & Redox Signaling·Anna KoniorTomasz J Guzik
Apr 30, 2014·Journal of Ophthalmology·Deli KrizovaPavel Studeny
May 2, 2014·Investigative Ophthalmology & Visual Science·Don YuenLu Chen
Nov 25, 2014·Journal of Leukocyte Biology·Holly R ChinneryPaul G McMenamin
Feb 6, 2015·The Journal of Physiology·Juri VogelKatrin Schröder

❮ Previous
Next ❯

Citations

Jun 3, 2020·Nature Communications·Anne WolfThomas Langmann
Oct 21, 2020·Investigative Ophthalmology & Visual Science·Nora Y HakamiHitesh M Peshavariya
May 12, 2021·Angiogenesis·Linxin ZhuGuei-Sheung Liu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.