NADPH-oxidase-derived ROS alters cell migration by modulating adhesions dynamics

Biology of the Cell
Maurício Tavares TamborindeguyMarcelo Lazzaron Lamers

Abstract

Cell migration requires the coordinated activation of structural and signalling molecules, such as the RhoGTPase Rac1. It is known that the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase complex assembly, which generates reactive oxygen species (ROS) at the cell membrane, also relies on Rac1 activation, indicating a possible effect of ROS during cell migration. In this study, we evaluated the effect of NADPH-oxidase-derived ROS on the migration process. Using time-lapse videos of CHO.K1 cells plated on fibronectin (2 μg/ml) or collagen (5 μg/cm2 ), we observed that depletion of ROS by N-acetyl-cysteine (NAC, 10 mM), an unspecific antioxidant, or diphenyliodonium (DPI, 10 μM), a NADPH-oxidase inhibitor, induced a ∼50% decrease in migration speed and severely impacted migration directionality. Then, we analysed the effects of NADPH oxidase on three migratory events: protrusion rate, adhesion process and signalling pathways related to cell migration. DPI induced an increase of ∼3 protrusion/cell, which were 2× faster but had a ∼50% retraction when compared with control. By pull-down assay, we observed no changes on Rac1 activation, indicating that ROS-mediated effects were related to downstream molecules, such as adhe...Continue Reading

References

Apr 6, 1995·Biochemical and Biophysical Research Communications·D IlicS Aizawa
Jul 1, 1994·American Journal of Respiratory Cell and Molecular Biology·J StolkA J Verhoeven
Dec 6, 2003·Science·Anne J RidleyAlan Rick Horwitz
Aug 1, 1993·Trends in Cell Biology·M D Schaller, J T Parsons
Jan 27, 2004·Nature Cell Biology·Donna J WebbAlan F Horwitz
Apr 26, 2006·The Journal of Biological Chemistry·Guangjie ChengJ David Lambeth
Aug 24, 2006·Science's STKE : Signal Transduction Knowledge Environment·Masuko Ushio-Fukai
Jun 25, 2008·The Journal of Cell Biology·Kai ChenJohn F Keaney
Aug 21, 2009·Nature·Matthias MachacekGaudenz Danuser
May 4, 2010·Free Radical Biology & Medicine·Elisa GiannoniPaola Chiarugi
Aug 24, 2010·Nature Reviews. Molecular Cell Biology·J Thomas ParsonsMartin A Schwartz
Jun 11, 2011·Journal of Cellular Physiology·Liv Ingrid FlinderEllen Skarpen
Dec 24, 2011·Journal of Cell Science·Miguel Vicente-Manzanares, Alan Rick Horwitz
Apr 16, 2014·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Yiping LiuXiaodong Chen
May 23, 2014·Current Biology : CB·Michael Schieber, Navdeep S Chandel
Jun 10, 2014·PloS One·Cristiane Ribeiro-PereiraChristina Barja-Fidalgo
Jul 6, 2014·Physiological Reviews·Dmitry B ZorovSteven J Sollott
Oct 12, 2014·Current Opinion in Cell Biology·Colleen R Reczek, Navdeep S Chandel
Feb 7, 2015·American Journal of Physiology. Renal Physiology·Yves GorinHanna E Abboud
Oct 9, 2015·Science Translational Medicine·Kristell Le GalMartin O Bergo
Nov 4, 2015·Current Protocols in Immunology·Warren Strober
Dec 5, 2015·Cell Metabolism·Maria Peiris-PagèsMichael P Lisanti
Jun 9, 2016·British Journal of Pharmacology·G TeixeiraJ M Wood
Jan 14, 2017·Molecular Oncology·Pahini PandyaVictoria Sanz-Moreno
Mar 31, 2017·The Journal of Experimental Biology·Daniel Munro, Jason R Treberg
Jun 18, 2017·Current Opinion in Cell Biology·Miranda V Hunter, Rodrigo Fernandez-Gonzalez

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Biophysics of Adhesion

Alterations in cell adhesion can disrupt important cellular processes and lead to a variety of diseases, including cancer and arthritis. It is also essential for infectious organisms, such as bacteria or viruses, to cause diseases. Understanding the biophysics of cell adhesion can help understand these diseases. Discover the latest research on the biophysics of adhesion here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.