Naegleria fowleri: a free-living highly pathogenic amoeba contains trypanothione/trypanothione reductase and glutathione/glutathione reductase systems

Experimental Parasitology
R N OndarzaE Hernández

Abstract

This paper presents definitive data showing that the thiol-bimane compound isolated and purified by HPLC from Naegleria fowleri trophozoites unequivocally corresponds by matrix assisted laser-desorption ionization-time-of-flight MS, to the characteristic monoprotonated ion of trypanothione-(bimane)(2) [M(+)H(+)] of m/z 1104.57 and to the trypanothione-(bimane) of m/z 914.46. The trypanothione disulfide T(S)(2) was also found to have a molecular ion of m/z 723.37. Additionally HPLC demonstrated that thiol-bimane compounds corresponding to cysteine and glutathione were present in Naegleria. The ion patterns of the thiol-bimane compounds prepared from commercial trypanothione standard, Entamoeba histolytica and Crithidia luciliae are identical to the Naegleria thiol-bimane compound. Partially purified extracts from N. fowleri showed the coexistence of glutathione and trypanothione reductases activities. There is not doubt that the thiol compound trypanothione, which was previously thought to occur only in Kinetoplastida, is also present in the human pathogens E. histolytica and N. fowleri, as well as in the non-pathogenic euglenozoan E. gracilis. The presence of the trypanothione/trypanothione reductase system in N. fowleri create...Continue Reading

References

Mar 23, 2002·The Journal of Eukaryotic Microbiology·Naomi M FastPatrick J Keeling
Nov 28, 2002·Biotechnology and Applied Biochemistry·Raúl N OndarzaGerardo Hurtado
Mar 13, 2003·Biotechnology and Applied Biochemistry·Raúl N OndarzaGerardo Hurtado
May 26, 2004·Biotechnology and Applied Biochemistry·Elsa María TamayoRaúl N Ondarza
Apr 2, 2005·Biotechnology and Applied Biochemistry·Raúl N OndarzaMathew Woolery

❮ Previous
Next ❯

Citations

Jul 5, 2011·International Journal for Parasitology·Fred R OpperdoesAloysius G M Tielens
Apr 9, 2008·Biochimica Et Biophysica Acta·R Luise Krauth-Siegel, Marcelo A Comini
Apr 9, 2008·Free Radical Biology & Medicine·Diego G AriasSergio A Guerrero
May 31, 2015·Plant Science : an International Journal of Experimental Plant Biology·Shun TamakiTakahiro Ishikawa
Mar 16, 2017·Environmental Science & Technology·Zhihao YuBrian H Clowers

❮ Previous
Next ❯

Related Concepts

Related Feeds

Amoebiasis

Amoebiasis, infection by the protozoan parasite Entamoeba histolytica, remains a global health problem, despite the availability of effective treatment. Here is the latest research.