NagR Differentially Regulates the Expression of the glmS and nagAB Genes Required for Amino Sugar Metabolism by Streptococcus mutans

Journal of Bacteriology
Lin Zeng, Robert A Burne

Abstract

The ability of bacteria to metabolize glucosamine (GlcN) and N-acetyl-d-glucosamine (GlcNAc) is considered important for persistent colonization of the oral cavity. In the dental caries pathogen Streptococcus mutans, the NagR protein regulates the expression of glmS, which encodes a GlcN-6-P synthetase, and nagA (GlcNAc-6-P deacetylase) and nagB (GlcN-6-P deaminase), which are required for the catabolism of GlcNAc and GlcN. Two NagR-binding sites (dre) were identified in each of the promoter regions for nagB and glmS. Using promoter-reporter gene fusions, the role of each dre site was examined in the regulation of glmS and nagB promoter activities in cells grown with glucose, GlcNAc, or GlcN. A synergistic relationship between the two dre sites in the glmS promoter that required proper spacing was observed, but that was not the case for nagB. Binding of purified NagR to DNA fragments from both promoter regions, as well as to dre sites alone, was strongly influenced by particular sugar phosphates. Using a random mutagenesis approach that targeted the effector-binding domain of NagR, mutants that displayed aberrant regulation of both the glmS and nagAB genes were identified. Collectively, these findings provide evidence that NagR...Continue Reading

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Citations

Jul 14, 2012·PloS One·Wei-Bing YangHuan-Zhong Shi
Dec 13, 2016·Inorganic Chemistry·Xiu-Ying ZhengLan-Sun Zheng
Dec 3, 2016·Frontiers in Cellular and Infection Microbiology·Muhammad AfzalOscar P Kuipers
Nov 26, 2020·Microorganisms·Puja YadavBarbara Spellerberg
Oct 25, 2020·Applied and Environmental Microbiology·Lulu ChenLin Zeng

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