PMID: 6410286Aug 18, 1983Paper

Na+/H+ exchange and cytoplasmic pH in the action of growth factors in human fibroblasts

Nature
W H MoolenaarS W de Laat

Abstract

The mechanisms by which growth factors stimulate metabolism and cell proliferation are largely unknown. Recent evidence suggests that mitogens rapidly activate a Na+/H+ exchange mechanism in the plasma membrane of their target cells, implicating cytoplasmic pH (pH1) as a potential 'messenger'. Indeed, growth stimulation of quiescent fibroblasts leads to intracellular alkalinization at approximately 1 h after mitogen addition, as measured by weak-acid distribution methods. We have used an internalized fluorescent pH1 indicator to examine the pH1-regulating mechanisms in diploid human fibroblasts and to obtain the first continuous pH1 recordings of the response to growth factors. We report here that (1) pH1 in human fibroblasts is controlled by a membrane-bound Na+/H+ exchanger, which rapidly restores pH1 after an acute cytoplasmic acidification, and (2) epidermal growth factor (EGF) and serum factors induce a rapid and persistent elevation of pH1 by modifying the pH1 sensitivity of the Na+H+ exchanger. We conclude that in addition to having a basic role in pH1 regulation, Na+/H+ exchange may function as a transmembrane signal transducer in the action of growth factors.

References

Jan 1, 1976·The Journal of General Physiology·W F Boron, P De Weer
Mar 1, 1977·The Journal of Physiology·R W Meech, R C Thomas
Feb 1, 1979·Developmental Biology·J L GraingerR A Steinhardt
Nov 1, 1981·The American Journal of Physiology·J L Kinsella, P S Aronson
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J PouysségurE Van Obberghen-Schilling
Oct 1, 1982·The Journal of Cell Biology·T J RinkT Pozzan
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·S Schuldiner, E Rozengurt
Jun 1, 1981·Journal of Cellular Physiology·M L Villereal
Apr 1, 1981·Physiological Reviews·A Roos, W F Boron
Jan 1, 1982·The American Journal of Physiology·M L FidelmanR D Moore
Mar 1, 1982·The American Journal of Physiology·D J Benos
Apr 1, 1981·The Journal of Cell Biology·C S RegulaR D Berlin

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Citations

Sep 30, 1999·Microscopy Research and Technique·G R Van Den BrinkM P Peppelenbosch
Mar 10, 2001·The Journal of Experimental Zoology·G A AhearnA Mandal
Oct 12, 2000·Proteins·G D BowmanC E Schutt
Aug 1, 1993·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J M Baltz
Feb 20, 1992·Biotechnology and Bioengineering·S S OzturkB O Palsson
Nov 1, 1990·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·T R Arnett, D W Dempster
Jan 1, 1995·Journal of Cellular Physiology·R HazanK L Crossin
Jan 1, 1987·Cancer Metastasis Reviews·J F WhitfieldP R Walker
Oct 1, 1984·Plant Cell Reports·V N RonchiG Luccarini
Mar 1, 1991·Pflügers Archiv : European journal of physiology·C A PollockD J Cockayne
Jan 1, 1991·Pflügers Archiv : European journal of physiology·D Günzel, S Galler
Apr 1, 1992·Pflügers Archiv : European journal of physiology·L WojnowskiH Oberleithner
May 1, 1987·Pflügers Archiv : European journal of physiology·M WeigtH Oberleithner
Dec 1, 1986·Journal of Bioenergetics and Biomembranes·I L SunH Löw
Jun 1, 1984·Journal of Bioenergetics and Biomembranes·I L SunC Grebing
Feb 1, 1996·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·P E RayM A Holliday
Jan 1, 1988·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·S A Mendoza
Dec 1, 1993·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·S H LarssonE Bratt
Jan 1, 1988·Archives of Virology·T Valyi-NagyT Albrecht
Sep 1, 1989·Klinische Wochenschrift·R Krapf
Jan 1, 1987·The Journal of Membrane Biology·E Padan, S Schuldiner
Jan 1, 1986·The Journal of Membrane Biology·S Grinstein, A Rothstein
Jan 1, 1986·The Journal of Membrane Biology·N Escobales, M Canessa
Jan 1, 1985·The Journal of Membrane Biology·T YadaW R Loewenstein
Dec 6, 2011·Pflügers Archiv : European journal of physiology·Mátyás CzepánPéter Hegyi
Feb 13, 1989·Biochimica Et Biophysica Acta·K MorganM A Mir
May 9, 1992·Biochimica Et Biophysica Acta·S ViniegraC A Rabito
Aug 30, 1989·Biochemical and Biophysical Research Communications·A MoranH Murer

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