NAIMA: target amplification strategy allowing quantitative on-chip detection of GMOs.

Nucleic Acids Research
Dany MorissetKristina Gruden

Abstract

We have developed a novel multiplex quantitative DNA-based target amplification method suitable for sensitive, specific and quantitative detection on microarray. This new method named NASBA Implemented Microarray Analysis (NAIMA) was applied to GMO detection in food and feed, but its application can be extended to all fields of biology requiring simultaneous detection of low copy number DNA targets. In a first step, the use of tailed primers allows the multiplex synthesis of template DNAs in a primer extension reaction. A second step of the procedure consists of transcription-based amplification using universal primers. The cRNA product is further on directly ligated to fluorescent dyes labelled 3DNA dendrimers allowing signal amplification and hybridized without further purification on an oligonucleotide probe-based microarray for multiplex detection. Two triplex systems have been applied to test maize samples containing several transgenic lines, and NAIMA has shown to be sensitive down to two target copies and to provide quantitative data on the transgenic contents in a range of 0.1-25%. Performances of NAIMA are comparable to singleplex quantitative real-time PCR. In addition, NAIMA amplification is faster since 20 min are s...Continue Reading

References

Mar 7, 1991·Nature·J Compton
Feb 9, 2002·Journal of Clinical Laboratory Analysis·P MarkoulatosM Moncany
Mar 6, 2002·Molecular Biotechnology·Birgit DeimanPeter Sillekens
May 7, 2003·Analytical and Bioanalytical Chemistry·Arne Holst-JensenKnut G Berdal
May 12, 2005·Journal of Agricultural and Food Chemistry·Andrea GerminiRosangela Marchelli
Jun 28, 2005·Nucleic Acids Research·Nicholas R Markham, Michael Zuker
Aug 17, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Gyorgy Csako
Jun 21, 2006·Plant Molecular Biology·Serge LeimanisJosé Remacle
Dec 20, 2007·BMC Biotechnology·Torstein TengsArne Holst-Jensen
Oct 3, 2009·Analytical and Bioanalytical Chemistry·Hans-Henno DörriesKornelia Berghof-Jäger

❮ Previous
Next ❯

Citations

Oct 13, 2009·Analytical and Bioanalytical Chemistry·David DobnikKristina Gruden
Oct 27, 2009·Analytical and Bioanalytical Chemistry·I-Jen LuTzu-Ming Pan
Jan 6, 2011·Applied Biochemistry and Biotechnology·Matthew B KerbyAnubhav Tripathi
Mar 9, 2013·Journal of Agricultural and Food Chemistry·Zhuoya ZhaoMi Ma
Mar 15, 2014·Journal of Agricultural and Food Chemistry·David DobnikMaja Ravnikar
Oct 29, 2009·Analytical and Bioanalytical Chemistry·Franz von Götz
Jul 3, 2010·Analytical and Bioanalytical Chemistry·Andreas MaderJürgen Rühe
Oct 31, 2009·Analytical and Bioanalytical Chemistry·Maddalena QuerciHermann Broll
Sep 4, 2014·Analytical and Bioanalytical Chemistry·Mojca MilavecJana Zel
Jan 20, 2012·BMC Biotechnology·Gabriella UjhelyiEsther J Kok
Feb 22, 2012·Biosensors & Bioelectronics·Kun ChenXiuping Wang
Nov 10, 2015·BioMed Research International·Marie-Alice FraitureNancy H Roosens
Dec 14, 2016·Water Research·Birgitte K Hønsvall, Lucy J Robertson
Dec 21, 2013·Analytical and Bioanalytical Chemistry·Fábio Cristiano Angonesi BrodEsther J Kok
Jul 1, 2014·Comprehensive Reviews in Food Science and Food Safety·Vincenzina Fusco, Grazia Marina Quero
Apr 17, 2010·Chemical Reviews·Chunsun Zhang, Da Xing
Feb 5, 2011·Analytical Chemistry·Jinchao GuoDabing Zhang

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
transgenic
phosphotransferase

Software Mentioned

NAIMA
ArrayPro Analyser®
SDS
Excel
Extra
NASBA Implemented Microarray Analysis ( NAIMA )
BLASTN
Co
Beacon Designer
DINAMelt

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.