Naive CD4+ T cells exhibit distinct expression patterns of cytokines and cell surface molecules on their primary responses to varying doses of antigen

The Journal of Immunology : Official Journal of the American Association of Immunologists
W IseS Kaminogawa

Abstract

The amount of an Ag used for stimulation affects the type and magnitude of T cell responses. In this study we have investigated the primary response of naive CD4(+) T cells derived from OVA-specific TCR-transgenic mice (OVA23-3) upon stimulation with varying doses of the antigenic peptide, OVA(323-339). IL-4 expression was maximal with 50 nM Ag and decreased significantly with increasing doses. In contrast, IFN-gamma expression, which was also detected at 50 nM Ag, increased with increasing doses. The expression patterns of mRNA for the Th2-specific transcription factors GATA-3 and c-Maf were parallel to that of IL-4. These expression profiles were not altered by the addition of anti-IL-4 plus anti-IL-12 mAbs, suggesting that cytokine receptor signaling is not essential. Naive CD4(+) T cells stimulated with 5 nM Ag elicited IgM secretion from cocultured B cells, whereas those stimulated with 50 nM Ag or more elicited apoptosis of B cells. This may be because at lower doses of Ag (5 nM), naive CD4(+) T cells express CD40 ligand and OX40, whereas at higher doses (50 nM), they express Fas ligand. Clearly, the expression of each type of molecule depends on the Ag dose, and different molecules had different expression patterns. Thus...Continue Reading

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Citations

Oct 23, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kazumi AsaiShuichi Kaminogawa
Mar 8, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sarita SehraAlexander L Dent
Oct 6, 2005·Blood·Lorenza CannarileCarlo Riccardi
Apr 9, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Gabriela PlesaLaurence C Eisenlohr
Aug 8, 2009·Bioscience, Biotechnology, and Biochemistry·Kiyoshi YamadaMamoru Totsuka
Oct 11, 2012·Bioscience, Biotechnology, and Biochemistry·Haruyo Nakajima-AdachiSatoshi Hachimura
Jun 9, 2020·Frontiers in Immunology·Nayan D Bhattacharyya, Carl G Feng
Dec 9, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Rodney MorganBaiyong Li
Jul 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Zsolt IllésVijay K Kuchroo
Aug 8, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Luisa CimminoKathryn L Calame
Jul 31, 2004·The Journal of Nutrition·Michele J Anderson, Kevin L Fritsche
Sep 21, 2005·The Journal of Experimental Medicine·Hidehiro YamaneWilliam E Paul
Oct 8, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marianne BoesHidde Ploegh
Aug 11, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kevin D Cook, Jim Miller
Apr 5, 2018·Immunology and Cell Biology·Janine GototChristian Kurts
Oct 22, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Wim JanssensJean-Marie R Saint-Remy
Jul 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Wataru IseSatoshi Hachimura
Jun 27, 2018·Immunological Reviews·Andrea J SantKatherine A Richards
Jun 6, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Scott N MuellerFrancis R Carbone

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