Na+/K+-ATPase α-subunit (nkaα) isoforms and their mRNA expression levels, overall Nkaα protein abundance, and kinetic properties of Nka in the skeletal muscle and three electric organs of the electric eel, Electrophorus electricus

PloS One
Biyun ChingYuen K Ip

Abstract

This study aimed to obtain the coding cDNA sequences of Na+/K+-ATPase α (nkaα) isoforms from, and to quantify their mRNA expression in, the skeletal muscle (SM), the main electric organ (EO), the Hunter's EO and the Sach's EO of the electric eel, Electrophorus electricus. Four nkaα isoforms (nkaα1c1, nkaα1c2, nkaα2 and nkaα3) were obtained from the SM and the EOs of E. electricus. Based on mRNA expression levels, the major nkaα expressed in the SM and the three EOs of juvenile and adult E. electricus were nkaα1c1 and nkaα2, respectively. Molecular characterization of the deduced Nkaα1c1 and Nkaα2 sequences indicates that they probably have different affinities to Na+ and K+. Western blotting demonstrated that the protein abundance of Nkaα was barely detectable in the SM, but strongly detected in the main and Hunter's EOs and weakly in the Sach's EO of juvenile and adult E. electricus. These results corroborate the fact that the main EO and Hunter's EO have high densities of Na+ channels and produce high voltage discharges while the Sach's EO produces low voltage discharges. More importantly, there were significant differences in kinetic properties of Nka among the three EOs of juvenile E. electricus. The highest and lowest Vmax...Continue Reading

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Mar 12, 2017·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Yau-Chung HuTsung-Han Lee
Nov 30, 2020·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Caryn Z PangShit F Chew

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Datasets Mentioned

BETA
KM282053

Methods Mentioned

BETA
electrophoresis
PCR
X-ray

Software Mentioned

Phylip
StepOne
MEMSAT3
ClustalX2
SPSS
MEMSAT
Bioedit
ExPASy Proteomic
PSIPRED
ImageJ

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