Na+/K+-ATPase α1 subunit, a novel therapeutic target for hepatocellular carcinoma

Oncotarget
Liping ZhuangPeiying Yang

Abstract

We aimed to identify the expression patterns of Na+/K+-ATPase (NKA) α subunits in human hepatocellular carcinoma (HCC) samples and evaluate these subunits as potential targets for HCC treatment. The mRNA expression profiles of NKA α subunits in human HCC samples were analyzed. We found that the mRNA expression for NKA α1 subunit (ATP1A1) was higher than that for other NKA α subunits. Also, ATP1A1 gene expression was markedly higher in HCC samples than in adjacent nontumor tissue samples. Western blotting data suggested that 6 of 14 (43%) HCC samples had elevated ATP1A1 protein expression. Furthermore, knockdown of ATP1A1 expression in human HCC HepG2 and MHCC97H cells markedly reduced their proliferation in vitro and suppressed the tumorigenicity of MHCC97H cells in vivo. Downregulation of ATP1A1 expression resulted in cell-cycle arrest at G2/M phase and apoptosis in HepG2 cells as well as decreased migration in Hep3B cells. We further validated that ATP1A1 downregulation caused intracellular accumulation of reactive oxygen species. Pretreatment with N-acetyl cysteine blocked cell-growth inhibition induced by ATP1A1 downregulation. Collectively, these data suggested that targeting ATP1A1 is a novel approach to the treatment of ...Continue Reading

References

Feb 17, 2001·Molecular Biology of the Cell·S A RajasekaranA K Rajasekaran
Jun 8, 2001·Anti-cancer Drugs·S JohanssonP Claeson
Oct 24, 2001·Cell·H VaziriR A Weinberg
Dec 26, 2001·Nucleic Acids Research·Ron EdgarAlex E Lash
Feb 7, 2003·American Journal of Physiology. Cell Physiology·Sigrid A RajasekaranAyyappan K Rajasekaran
Aug 2, 2003·American Journal of Physiology. Renal Physiology·Ayyappan K Rajasekaran, Sigrid A Rajasekaran
Jul 29, 2004·DNA Repair·Ari Barzilai, Ken-Ichi Yamamoto
Nov 17, 2007·The Journal of Urology·David B SeligsonAyyappan K Rajasekaran
Feb 28, 2008·Neurosurgery·Florence LefrancRobert Kiss
Jul 25, 2008·The New England Journal of Medicine·Josep M LlovetUNKNOWN SHARP Investigators Study Group
Feb 27, 2009·Journal of Cellular and Molecular Medicine·Véronique MathieuRobert Kiss
Sep 16, 2009·European Journal of Pharmacology·Christian HauckJochen Müller-Ehmsen
Oct 23, 2009·Nature·Stephen P Jackson, Jiri Bartek
Oct 12, 2010·Cell Cycle·Luis Miguel Guachalla, K Lenhard Rudolph
Oct 1, 2011·Liver International : Official Journal of the International Association for the Study of the Liver·Edmund Kwok-Kwan TungIrene Oi-Lin Ng
Dec 14, 2011·Structure·Chikashi ToyoshimaFlemming Cornelius
Jan 11, 2012·Oncology·Hee Chul ParkWon Young Tak
Mar 30, 2013·Anti-cancer Agents in Medicinal Chemistry·Petr BabulaRene Kizek
Jun 12, 2013·Investigational New Drugs·M SlingerlandH Gelderblom
Jun 27, 2013·Annals of Surgical Oncology·Ho-Yeong LimCheol-Keun Park
Jan 1, 2012·Hepatic Medicine : Evidence and Research·Renumathy DhanasekaranRoniel Cabrera

❮ Previous
Next ❯

Citations

May 10, 2017·Clinical Proteomics·Dan ZhangShufang Liang
Apr 6, 2019·Integrative Cancer Therapies·Peiying YangLorenzo Cohen
Mar 14, 2021·Biochimie·Camila Ignácio da SilvaPatrícia Burth
Mar 23, 2021·Acta Physiologica·Laura C KutzSandrine V Pierre
Jun 10, 2021·The Journal of Membrane Biology·Marina Marques ToledoLeandro A Barbosa
Jul 13, 2021·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Kei NakamuraEigo Otsuji
Oct 9, 2021·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Si TangWei Fan

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
xenograft
PCR

Software Mentioned

Cytoscape Plugin
ModFit
SPSS
Reactome FI

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis