PMID: 9159180May 27, 1997Paper

Na,K-ATPase subunit isoforms in human reticulocytes: evidence from reverse transcription-PCR for the presence of alpha1, alpha3, beta2, beta3, and gamma

Proceedings of the National Academy of Sciences of the United States of America
M K Stengelin, J F Hoffman

Abstract

The objective of this study has been to determine which Na,K-ATPase isoforms are expressed in red blood cells and whether kinetic differences in the uncoupled sodium efflux mode between the human red blood cell Na,K-ATPase and other preparations can be explained by differences in the underlying subunit composition. To this end, human reticulocyte RNA was isolated, reverse transcribed, amplified by PCR and appropriate primers, and sequenced. Primers from highly conserved areas as well as isoform-specific primers were used. The alpha1 and alpha3 isoforms of the alpha subunit, and the beta2 and beta3 isoforms of the beta subunit were found. The complete coding regions of the cDNAs for the reticulocyte subunits were sequenced from overlapping PCR fragments. No difference was found between the reticulocyte isoforms and the ones already known. The fact that we found beta2 but not beta1 in reticulocyte single-stranded cDNA, and beta1 but not beta2 in a leukocyte library indicates that leukocyte contamination of our reticulocyte preparation was negligible. Analysis of a human bone marrow library showed that alpha1, alpha2, and alpha3 as well as all three beta isoforms were present. The extent to which the kinetic properties of uncouple...Continue Reading

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Citations

Aug 5, 2004·Proceedings of the National Academy of Sciences of the United States of America·Joseph F HoffmanSulayman D Dib-Hajj
Feb 10, 2006·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Iva Dostanic-LarsonJerry B Lingrel
Oct 22, 2002·Proceedings of the National Academy of Sciences of the United States of America·Joseph F HoffmanDouglas R Yingst
Aug 8, 2007·Annual Review of Physiology·Joseph F Hoffman
May 13, 2010·Pflügers Archiv : European journal of physiology·Inna KaritskayaIrina Marakhova
Dec 28, 2006·Cell Biochemistry and Function·Silvana BalzanUmberto Montali
May 30, 2003·Proceedings of the National Academy of Sciences of the United States of America·Joseph F HoffmanAmittha Wickrema
Nov 14, 1998·The American Journal of Physiology·J MahaneyC Baylis
Mar 17, 2006·Biochimica Et Biophysica Acta·Fabio GrecoMauro Torti
Feb 24, 2009·Neuro-Chirurgie·P RigoardJ-M Maixent

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