PMID: 9421962Jan 9, 1998Paper

Nano- and microsecond time-resolved FTIR spectroscopy of the halorhodopsin photocycle

Photochemistry and Photobiology
A K Dioumaev, M S Braiman

Abstract

Step-scan Fourier transform infrared spectroscopy with 50 ns time resolution was applied to the early stages of the photocycle of halorhodopsin (hR) for the temperature range 3-42 degrees C. Kinetic data analysis with global fitting revealed two distinct kinetic processes associated with relaxations of the early red-shifted photoproduct hK; these processes have time constants tau 1 approximately equal to 280 ns and tau 2 approximately equal to 360 microns at 20 degrees C. Spectral features demonstrate that the tau 1 process corresponds to a transition between two distinct bathointermediates, hKE and hKL. The vibrational difference bands associated with both tau 1 and tau 2 transitions are spread throughout the whole 1800-900 cm-1 range. However, the largest bands correspond to ethylenic C=C stretches, fingerprint C-C stretches and hydrogen out-of-plane (HOOP) wags of the retinal chromophore. The time evolution of these difference bands indicate that both the tau 1 and tau 2 decay processes involve principally a relaxation of the chromophore and its immediate environment. The decay of the intense HOOP vibrations is nearly equally divided between the tau 1 and tau 2 processes, indicating a complex chromophore relaxation from a tw...Continue Reading

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Citations

Jan 28, 2003·Biophysical Journal·Vladislav BergoKenneth J Rothschild
Dec 22, 1999·Progress in Biophysics and Molecular Biology·J L Arrondo, F M Goñi
Sep 8, 1998·Current Opinion in Structural Biology·D Oesterhelt
Feb 9, 2010·The Journal of Physical Chemistry. B·Andrei K DioumaevJanos K Lanyi
Feb 13, 2009·The Journal of Biological Chemistry·Christoph PfistererStefan Fischer
Sep 10, 2014·The Journal of Physical Chemistry. B·Tino WolterAna-Nicoleta Bondar
Apr 20, 2005·Structure·Andreea D GruiaStefan Fischer
Jan 15, 2015·Journal of the American Chemical Society·Víctor A Lórenz-FonfríaJoachim Heberle
Oct 29, 2009·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Ionela RaduJoachim Heberle
Sep 14, 2000·Biochimica Et Biophysica Acta·G Váró
Sep 6, 2018·Journal of the American Chemical Society·Janne A IhalainenTilman Kottke
Feb 21, 2018·The Journal of Physical Chemistry. a·Misao MizunoYasuhisa Mizutani

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