Nano-Bio Interaction between Blood Plasma Proteins and Water-Soluble Silicon Quantum Dots with Enabled Cellular Uptake and Minimal Cytotoxicity

Nanomaterials
Shanmugavel ChinnathambiNaoto Shirahata

Abstract

A better understanding of the compatibility of water-soluble semiconductor quantum dots (QDs) upon contact with the bloodstream is important for biological applications, including biomarkers working in the first therapeutic spectral window for deep tissue imaging. Herein, we investigated the conformational changes of blood plasma proteins during the interaction with near-infrared light-emitting nanoparticles, consisting of Pluronic F127 shells and cores comprised of assembled silicon QDs terminated with decane monolayers. Albumin and transferrin have high quenching constants and form a hard protein corona on the nanoparticle. In contrast, fibrinogen has low quenching constants and forms a soft protein corona. A circular dichroism (CD) spectrometric study investigates changes in the protein's secondary and tertiary structures with incremental changes in the nanoparticle concentrations. As expected, the addition of nanoparticles causes the denaturation of the plasma proteins. However, it is noteworthy that the conformational recovery phenomena are observed for fibrinogen and transferrin, suggesting that the nanoparticle does not influence the ordered structure of proteins in the bloodstream. In addition, we observed enabled cellu...Continue Reading

References

Jul 18, 2002·Biochemistry·J K Amisha Kamal, Digambar V Behere
Dec 6, 2005·International Journal of Biological Macromolecules·Manas Kumar SantraDulal Panda
Apr 17, 2007·Journal of Colloid and Interface Science·Xing-Can ShenZuo-Yuan Wang
Aug 30, 2008·Nature Methods·Ute Resch-GengerThomas Nann
Sep 24, 2008·Proceedings of the National Academy of Sciences of the United States of America·Martin LundqvistKenneth A Dawson
Mar 5, 2011·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Bijan Kumar Paul, Nikhil Guchhait
May 7, 2011·ACS Medicinal Chemistry Letters·Chuqiao TuAngelique Y Louie
Aug 25, 2011·ACS Nano·Martin LundqvistKenneth Dawson
Nov 24, 2012·Chemical Society Reviews·Ken-Tye YongParas N Prasad
Jul 13, 2013·ACS Applied Materials & Interfaces·Guo-Hui PanRabah Boukherroub
Mar 26, 2014·Physical Chemistry Chemical Physics : PCCP·Surajit Chatterjee, Tushar Kanti Mukherjee
Mar 15, 2015·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Jyotsana PathakH B Bohidar
Mar 21, 2015·Biomacromolecules·Sandra RitzVolker Mailänder
Jun 4, 2015·Proceedings of the National Academy of Sciences of the United States of America·András MicsonaiJózsef Kardos
Mar 13, 2016·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Ziya SahinVeysel Kayser
Aug 27, 2016·Accounts of Chemical Research·Eric A OwensHak Soo Choi
Apr 5, 2017·Angewandte Chemie·Sourov ChandraFrançoise M Winnik
Apr 7, 2017·Nature Nanotechnology·Marilena Hadjidemetriou, Kostas Kostarelos
Jun 15, 2017·Journal of the American Chemical Society·Ivana MartinićStéphane Petoud
Mar 10, 2018·Journal of Controlled Release : Official Journal of the Controlled Release Society·Zahraa S Al-AhmadyKostas Kostarelos
Nov 30, 2018·Advanced Materials·Marilena HadjidemetriouKostas Kostarelos
Apr 27, 2019·Advanced Materials·Shoujun ZhuHongjie Dai
May 10, 2019·Science and Technology of Advanced Materials·Shanmugavel Chinnathambi, Naoto Shirahata
Aug 17, 2019·Nano Letters·Meshkat DinarvandSebastian Kruss
Feb 13, 2020·Nano Letters·Naoto ShirahataGeoffrey A Ozin
Jul 2, 2020·ACS Applied Materials & Interfaces·Yuanqiang SunZhaohui Li

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Citations

Sep 10, 2021·The Journal of Physical Chemistry Letters·Jingjian ZhouIlya Sychugov

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Methods Mentioned

BETA
circular dichroism
X-ray
light scattering
fluorescence microscopy
Assay
dynamic light scattering
size fractionation
Fluorescence

Software Mentioned

BeStSel
ASTRA

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